Extension of Sphingobium sp. BHC-A to a 2,4,5-trichlorophenoxyacetic acid mineralizing strain by metabolic engineering

• Published in: Journal of biotechnology 2013-07, Vol.166 (4), p.187-191
• Main Authors: Ge, Feng, Chen, Xu, Wang, Xin, Liao, Xuewei, Jiao, Yiying, Hong, Qing, Zhang, Longjiang, Wu, Jun
• Format: Article
• Language: English
• Subjects: 2,4,5-T; 2,4,5-Trichlorophenoxyacetic acid; 2,4,5-Trichlorophenoxyacetic Acid - metabolism; 2,5-Dichlorohydroquinone; Biodegradation, Environmental; biotechnology; genes; Homologous recombination; Hydroquinones - metabolism; lindane; Lindane - metabolism; metabolic engineering; Metabolic Engineering - methods; Metabolic Networks and Pathways - genetics; Mineralization; Minerals - metabolism; Models, Biological; proteins; Sphingomonadaceae - classification; Sphingomonadaceae - genetics; Sphingomonadaceae - metabolism; Sphingomonas; γ-Hexachlorocyclohexane
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2013.05.013

•Transfer of the upstream pathway for 2,4,5-T to 2,5-DCHQ into BHC-A.•Engineered strain BHC-Ae degraded 2,4,5-T to 2,5-DCHQ.•BHC-Ae mineralized 2,5-DCHQ through γ-HCH downstream degradation pathway. The gene cassette encoding for TftAB and TftCD proteins was integrated into the 16srDNA gene of the γ-hexachlorocyclohexane (γ-HCH) mineralizing strain Sphingobium sp. BHC-A by homologous recombination. The recombinant γ-HCH mineralizing strain may degrade 2,4,5-trichlorophenoxyacetic acid at a rate of 250nmol mg [protein]−1h−1, and the generated intermediate 2,5-dichlorohydroquinone may be further mineralized through γ-HCH downstream degradation pathway.