Stimulation of diploid fibroblast growth with serum-free medium conditioned by mezerein-treated monocytic leukemia cells

Medium conditioned by mezerein‐treated human acute monocytic leukemia cells (THP‐1) stimulated human fibroblast replication. Maximum mitogenic activity was elaborated by THP‐1 cells with a 24‐hr incubation in 10–7 M mezerein (activator phase) followed by a 36‐hr incubation in insulin‐supplemented se...

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Bibliographic Details
Published in:Journal of leukocyte biology 1984-05, Vol.35 (5), p.489-500
Main Authors: Gaffney, E V, Tsai, S C, Lingenfelter, S E, Dell'Aquila, M L, Gonda, J E
Format: Article
Language:English
Subjects:
Antineoplastic Agents, Phytogenic - pharmacology
Breast Neoplasms - physiopathology
Cell Division - drug effects
Cell Line
Diploidy
Diterpenes
fibroblast
Fibroblasts - drug effects
Fibroblasts - physiology
Growth Substances
Humans
Kinetics
Leukemia, Monocytic, Acute - physiopathology
Lung - embryology
macrophage
mezerein
monokine
Phorbol Esters - pharmacology
Phorbols - pharmacology
Platelet-Derived Growth Factor - pharmacology
Terpenes
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Summary:Medium conditioned by mezerein‐treated human acute monocytic leukemia cells (THP‐1) stimulated human fibroblast replication. Maximum mitogenic activity was elaborated by THP‐1 cells with a 24‐hr incubation in 10–7 M mezerein (activator phase) followed by a 36‐hr incubation in insulin‐supplemented serum‐free Roswell Park Memorial Institute (RPMI)‐1640 medium (effector phase). Growth stimulation was not due to the presence of residual mezerein. We previously reported that leukemia cells also produced a growth inhibitor. Fibroblast stimulation was resolved by isoelectrofocusing into several active fractions separate from the growth inhibitory activity for malignant mammary cells. Conditioned medium was mitogenic for fibroblasts in the presence of high concentrations of fetal bovine and human whole blood sera. Growth stimulation was observed in plasma‐derived serum only when supplemented with exogenous platelet‐derived growth factor. Thus, this THP‐1 cell product does not fulfill the role of a competence factor.
ISSN:0741-5400
1938-3673
DOI:10.1002/jlb.35.5.489