Comparison of ChromID C. difficile agar and cycloserine-cefoxitin-fructose agar for the recovery of Clostridium difficile

The rapidly changing epidemiology of Clostridium difficile infection highlights the need for improved and continuing surveillance involving stool culturing to enable molecular tracking. Culture of C. difficile can be difficult and time consuming. In this report ChromID C. difficile agar (CDIF) was c...

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Bibliographic Details
Published in:Pathology 2013-08, Vol.45 (5), p.495-500
Main Authors: Boseiwaqa, Lusiana V., Foster, Niki F., Thean, Sara K., Squire, Michele M., Riley, Thomas V., Carson, Kerry C.
Format: Article
Language:English
Subjects:
Agar - chemistry
Agar - classification
Cefoxitin - analysis
chromID
chromogenic agar
clostridium difficile
Clostridium difficile - growth & development
Clostridium difficile - isolation & purification
Cycloserine - analysis
Enterocolitis, Pseudomembranous - diagnosis
Enterocolitis, Pseudomembranous - epidemiology
Ethanol
Fructose - analysis
Humans
isolation medium
Microbiological Techniques - methods
Ribotyping
Temperature
Time Factors
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Summary:The rapidly changing epidemiology of Clostridium difficile infection highlights the need for improved and continuing surveillance involving stool culturing to enable molecular tracking. Culture of C. difficile can be difficult and time consuming. In this report ChromID C. difficile agar (CDIF) was compared to cycloserine-cefoxitin-fructose-egg- yolk agar which contained 0.1% sodium taurocholate (TCCFA) as a germinant. All ribotypes of C. difficile tested (n= 90) grew well on CDIF within 24h and most gave characteristic small irregular black colonies with a raised umbonate profile. Counts from standard suspensions of C. difficile at 24h (P
ISSN:0031-3025
1465-3931
DOI:10.1097/PAT.0b013e3283632680