Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea

A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the l...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 1984-01, Vol.96 (1), p.35-40
Main Authors: LIN, Jung-Yaw, CHOU, Tze-Bin
Format: Article
Language:English
Subjects:
Agaricales - analysis
Amino Acids - analysis
Analytical, structural and metabolic biochemistry
Animals
Antineoplastic Agents, Phytogenic
antitumor antibiotics
Anura
Biological and medical sciences
Cats
Chemical Phenomena
Chemistry
Chickens
Dogs
Ducks
Electrophoresis, Disc
Fundamental and applied biological sciences. Psychology
Guinea Pigs
Hemagglutination Tests
Humans
In Vitro Techniques
lectins
Lectins - isolation & purification
Lectins - pharmacology
Lectins - toxicity
Lethal Dose 50
Mice
Miscellaneous
Molecular Weight
Peptide Fragments - analysis
Proteins
Rats
Sarcoma 180 - therapy
Trypsin
Volvariella volvacea
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.
ISSN:0021-924X
1756-2651
DOI:10.1093/oxfordjournals.jbchem.a134826