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Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea
A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the l...
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| Published in: | Journal of biochemistry (Tokyo) 1984-01, Vol.96 (1), p.35-40 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c491t-136fd6bff20c0d5945c57ad96891d3aa2c9cf2d9025e5ca85816316e622711bd3 |
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| container_end_page | 40 |
| container_issue | 1 |
| container_start_page | 35 |
| container_title | Journal of biochemistry (Tokyo) |
| container_volume | 96 |
| creator | LIN, Jung-Yaw CHOU, Tze-Bin |
| description | A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells. |
| doi_str_mv | 10.1093/oxfordjournals.jbchem.a134826 |
| format | article |
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The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.</description><identifier>ISSN: 0021-924X</identifier><identifier>EISSN: 1756-2651</identifier><identifier>DOI: 10.1093/oxfordjournals.jbchem.a134826</identifier><identifier>PMID: 6541650</identifier><identifier>CODEN: JOBIAO</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Agaricales - analysis ; Amino Acids - analysis ; Analytical, structural and metabolic biochemistry ; Animals ; Antineoplastic Agents, Phytogenic ; antitumor antibiotics ; Anura ; Biological and medical sciences ; Cats ; Chemical Phenomena ; Chemistry ; Chickens ; Dogs ; Ducks ; Electrophoresis, Disc ; Fundamental and applied biological sciences. Psychology ; Guinea Pigs ; Hemagglutination Tests ; Humans ; In Vitro Techniques ; lectins ; Lectins - isolation & purification ; Lectins - pharmacology ; Lectins - toxicity ; Lethal Dose 50 ; Mice ; Miscellaneous ; Molecular Weight ; Peptide Fragments - analysis ; Proteins ; Rats ; Sarcoma 180 - therapy ; Trypsin ; Volvariella volvacea</subject><ispartof>Journal of biochemistry (Tokyo), 1984-01, Vol.96 (1), p.35-40</ispartof><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c491t-136fd6bff20c0d5945c57ad96891d3aa2c9cf2d9025e5ca85816316e622711bd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9711019$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6541650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LIN, Jung-Yaw</creatorcontrib><creatorcontrib>CHOU, Tze-Bin</creatorcontrib><title>Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea</title><title>Journal of biochemistry (Tokyo)</title><addtitle>J Biochem</addtitle><description>A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.</description><subject>Agaricales - analysis</subject><subject>Amino Acids - analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Antineoplastic Agents, Phytogenic</subject><subject>antitumor antibiotics</subject><subject>Anura</subject><subject>Biological and medical sciences</subject><subject>Cats</subject><subject>Chemical Phenomena</subject><subject>Chemistry</subject><subject>Chickens</subject><subject>Dogs</subject><subject>Ducks</subject><subject>Electrophoresis, Disc</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Guinea Pigs</subject><subject>Hemagglutination Tests</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>lectins</subject><subject>Lectins - isolation & purification</subject><subject>Lectins - pharmacology</subject><subject>Lectins - toxicity</subject><subject>Lethal Dose 50</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Molecular Weight</subject><subject>Peptide Fragments - analysis</subject><subject>Proteins</subject><subject>Rats</subject><subject>Sarcoma 180 - therapy</subject><subject>Trypsin</subject><subject>Volvariella volvacea</subject><issn>0021-924X</issn><issn>1756-2651</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><recordid>eNpVkFuL1DAYhoMo67j6E4RcqFd2zKFJmwsvZFidhRERdnXwJnzNgcnYNrtJu6z-eju0DHiVw_t8ycuD0FtK1pQo_iE--pjsMY6phzavj405uG4NlJc1k0_QilZCFkwK-hStCGG0UKzcP0cvcj6ejozzC3QhRUmlICt0e51jC0OIPYbe4s0BEpjBpfB3voweA945M4Qe-xQ7fGVD0zr8dcyHFGP3Hv-I7QOk4NoW8MNpbxy8RM_8VM69WtZLdPv56mazLXbfvlxvPu0KUyo6FJRLb2XjPSOGWKFKYUQFVslaUcsBmFHGM6sIE04YqEVNJafSScYqShvLL9G7-d27FO9HlwfdhWxOVXoXx6xpSSivVTmBH2fQpJhzcl7fpdBB-qMp0Set-n-tetaqF63T_Ovlo7HpnD1PLx6n_M2SQzbQ-gS9CfmMqakuoWrCihkLeXCP5xjSby0rXgm93f_S32_YT7Lfllrwf6Iml_I</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>LIN, Jung-Yaw</creator><creator>CHOU, Tze-Bin</creator><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>19840101</creationdate><title>Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea</title><author>LIN, Jung-Yaw ; CHOU, Tze-Bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c491t-136fd6bff20c0d5945c57ad96891d3aa2c9cf2d9025e5ca85816316e622711bd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Agaricales - analysis</topic><topic>Amino Acids - analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Antineoplastic Agents, Phytogenic</topic><topic>antitumor antibiotics</topic><topic>Anura</topic><topic>Biological and medical sciences</topic><topic>Cats</topic><topic>Chemical Phenomena</topic><topic>Chemistry</topic><topic>Chickens</topic><topic>Dogs</topic><topic>Ducks</topic><topic>Electrophoresis, Disc</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Guinea Pigs</topic><topic>Hemagglutination Tests</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>lectins</topic><topic>Lectins - isolation & purification</topic><topic>Lectins - pharmacology</topic><topic>Lectins - toxicity</topic><topic>Lethal Dose 50</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>Molecular Weight</topic><topic>Peptide Fragments - analysis</topic><topic>Proteins</topic><topic>Rats</topic><topic>Sarcoma 180 - therapy</topic><topic>Trypsin</topic><topic>Volvariella volvacea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LIN, Jung-Yaw</creatorcontrib><creatorcontrib>CHOU, Tze-Bin</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of biochemistry (Tokyo)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LIN, Jung-Yaw</au><au>CHOU, Tze-Bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea</atitle><jtitle>Journal of biochemistry (Tokyo)</jtitle><addtitle>J Biochem</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>96</volume><issue>1</issue><spage>35</spage><epage>40</epage><pages>35-40</pages><issn>0021-924X</issn><eissn>1756-2651</eissn><coden>JOBIAO</coden><abstract>A lectin was purified from edible mushroom, Volvariella volvacea by extraction with 5% cold acetic acid in the presence of 0.1 % 2-mercaptoethanol, followed by ammonium sulfate fractionation, and DEAE-C-52 and CM-C-52 column chromatographies. The molecular weight was measured to be 26,000, and the lectin consisted of two non-identical subunits as demonstrated by gel filtration and polyacrylamide gel electrophoresis. The lectin does not contain half-cystine, methionine, or histidine. The LD50 of the lectin is 17.5 mg per kg body weight of mice. The lectin has a moderate inhibitory effect on the growth of tumor cells.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>6541650</pmid><doi>10.1093/oxfordjournals.jbchem.a134826</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-924X |
| ispartof | Journal of biochemistry (Tokyo), 1984-01, Vol.96 (1), p.35-40 |
| issn | 0021-924X 1756-2651 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_14013894 |
| source | JSTAGE; Oxford University Press Archive |
| subjects | Agaricales - analysis Amino Acids - analysis Analytical, structural and metabolic biochemistry Animals Antineoplastic Agents, Phytogenic antitumor antibiotics Anura Biological and medical sciences Cats Chemical Phenomena Chemistry Chickens Dogs Ducks Electrophoresis, Disc Fundamental and applied biological sciences. Psychology Guinea Pigs Hemagglutination Tests Humans In Vitro Techniques lectins Lectins - isolation & purification Lectins - pharmacology Lectins - toxicity Lethal Dose 50 Mice Miscellaneous Molecular Weight Peptide Fragments - analysis Proteins Rats Sarcoma 180 - therapy Trypsin Volvariella volvacea |
| title | Isolation and Characterization of a Lectin from Edible Mushroom, Volvariella volvacea |
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