The recognition sequence of site-specific endonuclease BbvII from Bacillus brevis 80

Site-specific endonuclease BbvII from Bacillus brevis 80 recognizes the non-symmetrical hexanucleotide and cleaves DNA at distances of 2 and 6 nucleotides from the recognition site: 5'-GAAGAC2N↓ 3'-CTTCTG6N↑

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Bibliographic Details
Published in:FEBS letters 1984-01, Vol.177 (1), p.23-26
Main Authors: Matvienko, N.I., Pachkunov, D.M., Kramarov, V.M.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Bacillus brevis
Bacillus brevis Site-specific endonuclease Molecular cloning
Bacillus brevis Site-specific endonucleaseMolecular cloning
Biological and medical sciences
Biotechnology
endodeoxyribonuclease BbvII
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Hydrolases
Methods. Procedures. Technologies
nucleotide sequence
This enzyme may be used in molecular cloning for vectors with multiple restriction sites
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Description
Summary:Site-specific endonuclease BbvII from Bacillus brevis 80 recognizes the non-symmetrical hexanucleotide and cleaves DNA at distances of 2 and 6 nucleotides from the recognition site: 5'-GAAGAC2N↓ 3'-CTTCTG6N↑
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(84)80973-4