Isolation and characteristics of a short rod‐shaped mutant of Pseudomonas aeruginosa

Among 40 short rod‐shaped mutants of Pseudomonas aeruginosa PAO isolated after nitrosoguanidine mutagenesis, a stable clone designated KG1034 was obtained and studied for its cellular and genetical features. Cells of the parent strain, PAO2302 had a mean cell length of 1.9 μm, whereas that of KG1034...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 1984-01, Vol.24 (2‐3), p.345-349
Main Authors: Gotoh, Naomasa, Hanehara, Chieko, Tanino, Teruo
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
cell length
Fundamental and applied biological sciences. Psychology
genetical mapping
growth curve
Isolation of short rod mutant
Microbiology
Morphology, structure, chemical composition
penicillin‐binding proteins
Pseudomonas aeruginosa
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Among 40 short rod‐shaped mutants of Pseudomonas aeruginosa PAO isolated after nitrosoguanidine mutagenesis, a stable clone designated KG1034 was obtained and studied for its cellular and genetical features. Cells of the parent strain, PAO2302 had a mean cell length of 1.9 μm, whereas that of KG1034 was 1.3 μm. The doubling time of KG1034 was less than that of the parent strain, although both strains elongated at the same rate and exhibited the same relative amounts and pattern of penicillin‐binding proteins. These results suggested that the phenotype of KG1034 was due to the initiation of septation at an earlier stage. The new gene responsible for this phenotype was named srs (abbreviation for short rod shape) and mapped by conjugation between cys‐54 and puuC.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1984.tb01332.x