Use of Lemna minor L. as a bioassay in allelopathy

Investigations in allelopathy often require the use of a bioassay for evaluating limited quantities of potentially active growth regulators. A bioassay procedure was developed using L. minor grown in 1.5-ml aliquots of nutrient medium with and without allelochemicals in wells of 24-well tissue cultu...

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Bibliographic Details
Published in:Journal of chemical ecology 1985-01, Vol.11 (1), p.65-72
Main Authors: Einhellig, F A, Leather, G R, Hobbs, L L
Format: Article
Language:English
Subjects:
Freshwater
Lemna minor
Online Access:Get full text
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Summary:Investigations in allelopathy often require the use of a bioassay for evaluating limited quantities of potentially active growth regulators. A bioassay procedure was developed using L. minor grown in 1.5-ml aliquots of nutrient medium with and without allelochemicals in wells of 24-well tissue culture cluster dishes with loose-fitting lids. Tests using six replications per treatment with several flavonoid compounds and derivatives of coumarin, benzoic acid, and cinnamic acid demonstrated that the bioassay was capable of measuring inhibition at levels of compound ranging from 50 to 1000 mu mol. Strongly inhibitory treatments were visible after 1 or 2 days. After 7 days of growth, frond number, growth rate, and dry weight were used to evaluate effects.
ISSN:0098-0331
DOI:10.1007/BF00987606