Influence of hPTH(1-34), hCT and prostaglandins on in vitro metabolism of human trabecular bone

• Published in: Calcified tissue international 1983-01, Vol.35 (4-5)
• Main Authors: Keck, E, Schartl, A, West, T B, Krueskemper, H L
• Format: Article
• Language: English
• ISSN: 0171-967X

A procedure for incubating trabeculae from the femoral heads of human hip replacement recipients has allowed us to investigate the metabolism of Ca super(2) Mg super(2+) and PO sub(4) super(3-) by human bone. As compared to controls addition of hPTH(1-34) caused a dose- and time-dependent decrease in the net uptake of Ca super(2+) and Mg super(2+), most probably due to an increase in the release of these electrolytes from the bone, while PO sub(4) super(3-) concentration and alkaline phosphatase (AP) activity remained unchanged. hPTH(3-34) was ineffective in this system. Upon addition of syn hCT there was a dose- and time-dependent increase in the net uptake of Ca super(2+) and Mg super(2+) out of the incubation medium, which is most probably due to inhibition of electrolyte release from the bone. The effects of the prostaglandins PGE sub(2) and PGF sub(2 alpha ) on the net electrolyte movements were similar to that of hPTH(1-34). The effect of PGF was only half as great as that of PGE sub(2), whereas PGA sub(1) and PGA sub(2) had no significant effect.