Development of test strips for rapid buprenorphine detection in vitro

Buprenorphine (BUP) is the primary treatment for narcotic addiction, but it is often abused by opioid-dependent patients in many countries. For timely and effective detection and controlling the amount of BUP used in therapy, a rapid and sensitive test is needed. In the present study, we describe th...

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Bibliographic Details
Published in:Clinical biochemistry 2013-08, Vol.46 (12), p.1093-1098
Main Authors: Li, Sheng-Jin, Wang, Ding, Zhang, Jun, Zheng, Jian, Chen, Wei-Xian
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Buprenorphine
Buprenorphine - urine
Chemistry, Clinical - methods
Chromatography, Affinity
Colloidal gold
Electrophoresis, Polyacrylamide Gel
Gravitation
Hydrogen-Ion Concentration
Limit of Detection
Mice
Mice, Inbred BALB C
Monoclonal antibody
Reagent Strips
Test strips
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Summary:Buprenorphine (BUP) is the primary treatment for narcotic addiction, but it is often abused by opioid-dependent patients in many countries. For timely and effective detection and controlling the amount of BUP used in therapy, a rapid and sensitive test is needed. In the present study, we describe the development of test strips using monoclonal antibodies (MAbs) for the detection of BUP. The MAbs were generated from hybridomas, and purified MAbs were used to create colloidal gold–antibody conjugates that were placed in the test strips. The BUP test strips had a limit of detection (LOD) of 12.5ng/mL and did not cross-react with other drugs tested at physiological levels. Therefore, this assay has sufficient sensitivity and specificity for BUP detection in urine specimens so that the dosage of BUP given to individuals being treated for opioid dependence can be monitored. •We developed various monoclonal antibody against buprenorphine by hybridoma technique.•A test strip for rapid detection of buprenorphine was generated.•The sensitivity, specificity and precision of the strip were assessed.
ISSN:0009-9120
1873-2933
DOI:10.1016/j.clinbiochem.2013.05.001