Pyruvate Promotion of DNA Synthesis in Serum-Free Primary Cultures of Adult Rat Hepatocytes

Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloac...

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Bibliographic Details
Published in:In Vitro 1983-03, Vol.19 (3), p.159-166
Main Authors: McGowan, J A, Bucher, N L
Format: Article
Language:English
Subjects:
Alanine - pharmacology
Amino acids
Animals
Autoradiography
Cell growth
Cells
Cells, Cultured
Cultured cells
DNA
DNA - analysis
DNA - biosynthesis
Drug Synergism
Epidermal Growth Factor - pharmacology
Hepatocytes
Insulin
Insulin - pharmacology
Lactates
Liver - metabolism
Liver cells
Pyruvates - pharmacology
Rats
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Summary:Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloacetate, α-ketoglutarate, α-ketobutyrate, succinate, fumarate, and malate), though less potent than pyruvate and lactate, also elevate DNA synthesis, whereas alanine at similar concentrations is inhibitory.
ISSN:0073-5655
1475-2689
DOI:10.1007/bf02618054