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Pyruvate Promotion of DNA Synthesis in Serum-Free Primary Cultures of Adult Rat Hepatocytes

Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloac...

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Published in:	In Vitro 1983-03, Vol.19 (3), p.159-166
Main Authors:	McGowan, J A, Bucher, N L
Format:	Article
Language:	English
Subjects:	Alanine - pharmacology Amino acids Animals Autoradiography Cell growth Cells Cells, Cultured Cultured cells DNA DNA - analysis DNA - biosynthesis Drug Synergism Epidermal Growth Factor - pharmacology Hepatocytes Insulin Insulin - pharmacology Lactates Liver - metabolism Liver cells Pyruvates - pharmacology Rats
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container_title	In Vitro
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description	Pyruvate (2 to 60 mM), acting alone and in conjunction with insulin and epidermal growth factor (EGF), enhances DNA synthesis in primary monolayer cultures of adult rat liver parenchymal cells. Lactate can replace pyruvate in stimulating DNA synthesis. Several other intermediary metabolites (oxaloacetate, α-ketoglutarate, α-ketobutyrate, succinate, fumarate, and malate), though less potent than pyruvate and lactate, also elevate DNA synthesis, whereas alanine at similar concentrations is inhibitory.
doi_str_mv	10.1007/bf02618054
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Lactate can replace pyruvate in stimulating DNA synthesis. 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source	JSTOR Archival Journals and Primary Sources Collection; Springer Online Journal Archives (Through 1996)
subjects	Alanine - pharmacology Amino acids Animals Autoradiography Cell growth Cells Cells, Cultured Cultured cells DNA DNA - analysis DNA - biosynthesis Drug Synergism Epidermal Growth Factor - pharmacology Hepatocytes Insulin Insulin - pharmacology Lactates Liver - metabolism Liver cells Pyruvates - pharmacology Rats
title	Pyruvate Promotion of DNA Synthesis in Serum-Free Primary Cultures of Adult Rat Hepatocytes
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