Diversity, abundance, and distribution of NO-forming nitrite reductase-encoding genes in deep-sea subsurface sediments of the South China Sea

• Published in: Geobiology 2013-03, Vol.11 (2), p.170-179
• Main Authors: Li, M., Hong, Y., Cao, H., Klotz, M. G., Gu, J.-D.
• Format: Article
• Language: English
• Subjects: Bacteria - classification; Bacteria - enzymology; Bacteria - genetics; Biota; China; Cluster Analysis; Genetic Variation; Geologic Sediments - microbiology; Molecular Sequence Data; Nitrite Reductases - genetics; Phylogeny; Sequence Analysis, DNA
• ISSN: 1472-4677; 1472-4669
• DOI: 10.1111/gbi.12020

In marine ecosystems, both nitrite‐reducing bacteria and anaerobic ammonium‐oxidizing (anammox) bacteria, containing different types of NO‐forming nitrite reductase–encoding genes, contribute to the nitrogen cycle. The objectives of study were to reveal the diversity, abundance, and distribution of NO‐forming nitrite reductase–encoding genes in deep‐sea subsurface environments. Results showed that higher diversity and abundance of nirS gene than nirK and Scalindua‐nirS genes were evident in the sediments of the South China Sea (SCS), indicating bacteria containing nirS gene dominated the NO‐forming nitrite‐reducing microbial community in this ecosystem. Similar diversity and abundance distribution patterns of both nirS and Scalindua‐nirS genes were detected in this study sites, but different from nirK gene. Further statistical analyses also showed both nirS and Scalindua‐nirS genes respond similarly to environmental factors, but differed from nirK gene. These results suggest that bacteria containing nirS and Scalindua‐nirS genes share similar niche in deep‐sea subsurface sediments of the SCS, but differed from those containing nirK gene, indicating that community structures of nitrite‐reducing bacteria are segregated by the functional modules (NirS vs. NirK) rather than the competing processes (anammox vs. classical denitrification).