Genetic characterization, at the mitochondrial and nuclear DNA levels, of five Canary Island dog breeds

Summary Many studies presenting genetic analysis of dog breeds have been conducted without the inclusion of island dog breeds, although isolation can be one of the main factors in their origin. Here we report the genetic analysis at the nuclear and mitochondrial DNA levels of five Canary Island dog...

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Bibliographic Details
Published in:Animal genetics 2013-08, Vol.44 (4), p.432-441
Main Authors: Suárez, N. M., Betancor, E., Fregel, R., Pestano, J.
Format: Article
Language:English
Subjects:
Alleles
Animals
Base Sequence
Breeding
Breeding of animals
Canary archipelago
Canis lupus familiaris
Cluster Analysis
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - genetics
DNA Primers - genetics
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - genetics
Dogs
Dogs - classification
Dogs - genetics
Genetic Markers
Genetic Variation
Genetics, Population
Haplotypes
Microsatellite Repeats - genetics
microsatellites
mitochondrial DNA
Molecular Sequence Data
Phylogeny
Sequence Alignment - veterinary
Sequence Analysis, DNA - veterinary
Spain
Species Specificity
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Summary:Summary Many studies presenting genetic analysis of dog breeds have been conducted without the inclusion of island dog breeds, although isolation can be one of the main factors in their origin. Here we report the genetic analysis at the nuclear and mitochondrial DNA levels of five Canary Island dog breeds (Canarian Warren Hound, Canary Island Mastiff, Garafiano Shepherd, La Palma Rat‐Hunter and El Hierro Wolfhound) to fill this gap and, at the same time, genetically characterize these breeds. We identified 168 alleles in autosomal microsatellites and 16 mitochondrial haplotypes. Observed and expected heterozygosities ranged from 0.556 to 0.783 and from 0.737 to 0.943 respectively. Furthermore, three haplotypes were newly described and exclusive to a particular breed (A17+ in the Canary Island Mastiff; A33+ in the Canarian Warren Hound; Bi in the La Palma Rat‐Hunter). The outcome of our analyses also revealed different breed histories consistent with historical documents and hypothetical origin designations. Although mtDNA haplotypes showed poor breed discriminating power, autosomal markers allowed a clear clustering of each single population. We expect that our results, together with further analyses, will help to make the population histories of island dog breeds clearer.
ISSN:0268-9146
1365-2052
DOI:10.1111/age.12024