Identification and characterisation of side population cells in the canine pituitary gland

To date, stem/progenitor cells have not been identified in the canine pituitary gland. Cells that efficiently exclude the vital dye Hoechst 33342 can be visualised and identified using fluorescence activated cell sorting (FACS) as a ‘side population’ (SP), distinct from the main population (MP). Suc...

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Bibliographic Details
Published in:The veterinary journal (1997) 2012-06, Vol.192 (3), p.476-482
Main Authors: van Rijn, Sarah J., Gremeaux, Lies, Riemers, Frank M., Brinkhof, Bas, Vankelecom, Hugo, Penning, Louis C., Meij, Björn P.
Format: Article
Language:English
Subjects:
Animals
anterior pituitary
Dog
Dogs
flow cytometry
Flow Cytometry - veterinary
Gene Expression Regulation - physiology
Pituitary gland
Pituitary Gland - cytology
Pituitary Gland - physiology
pro-opiomelanocortin
Progenitor
quantitative polymerase chain reaction
Side population
Side-Population Cells - cytology
Side-Population Cells - physiology
Stem cell
stem cells
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Summary:To date, stem/progenitor cells have not been identified in the canine pituitary gland. Cells that efficiently exclude the vital dye Hoechst 33342 can be visualised and identified using fluorescence activated cell sorting (FACS) as a ‘side population’ (SP), distinct from the main population (MP). Such SPs have been identified in several tissues and display stem/progenitor cell characteristics. In this study, a small SP (1.3%, n=6) was detected in the anterior pituitary glands of healthy dogs. Quantitative PCR indicated significantly higher expression of CD34 and Thy1 in this SP, but no differences in the expression of CD133, Bmi-1, Axin2 or Shh. Pro-opiomelanocortin (POMC) and Lhx3 expression were significantly higher in the MP than in the SP, but no differences in the expression of Tpit, GH or PRL were found. The study demonstrated the existence of an SP of cells in the normal canine pituitary gland, encompassing cells with stem cell characteristics and without POMC expression.
ISSN:1090-0233
1532-2971
DOI:10.1016/j.tvjl.2011.06.030