Role of microRNA-21 in regulating 3T3-L1 adipocyte differentiation and adiponectin expression

MicroRNAs are endogenous small RNAs with a high degree of conservation, participating in a variety of vital activities. In present study, to explore the effect of microRNAs on 3T3-L1 adipocyte differentiation and adiponectin expression, the adipo-related microRNAs were screened and identified by mic...

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Bibliographic Details
Published in:Molecular biology reports 2013-08, Vol.40 (8), p.5027-5034
Main Authors: Kang, Min, Yan, Li-Mei, Zhang, Wen-Yu, Li, Yu-Mei, Tang, An-Zhou, Ou, He-Sheng
Format: Article
Language:English
Subjects:
3T3-L1 Cells
Adipocytes
Adipocytes - metabolism
Adipocytes - physiology
Adiponectin - metabolism
Animal Anatomy
Animal Biochemistry
Animals
Biomedical and Life Sciences
Cell Differentiation - genetics
Cell Differentiation - physiology
DNA Primers - genetics
Gene expression
Gene Expression Profiling
Gene Expression Regulation - genetics
Histology
Life Sciences
Mice
Microarray Analysis
MicroRNAs
MicroRNAs - metabolism
Molecular biology
Morphology
Proteins
Transcription Factor AP-1 - metabolism
Transfection
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Summary:MicroRNAs are endogenous small RNAs with a high degree of conservation, participating in a variety of vital activities. In present study, to explore the effect of microRNAs on 3T3-L1 adipocyte differentiation and adiponectin expression, the adipo-related microRNAs were screened and identified by micorRNA microarray. The highly expression plasmid of microRNA-21 with obvious expression up-regulation (miR-21) and its anti-sense (miR-21 inhibitor) were constructed and transfected into 3T3-L1 preadipocytes. The effect of miR-21 on 3T3-L1 adipocyte differentiation was observed, and the protein and mRNA expression level of adiponectin and AP-1 were analyzed. Results showed that, the expression profiles of microRNAs significantly changed during 3T3-L1 adipocyte differentiation. The expression of miR-21 was obviously up-regulated. miR-21 could significantly promote adipocyte differentiation, increase adiponectin mRNA and protein expression, while decrease AP-1 protein level. Meanwhil, miR-21 inhibitor blocked the effects of miR-21 mentioned above. The overexpression of AP-1 could absolutely reverse the stimulatory effect of miR-21 on adiponectin. miR-21 plays an important role in regulating adipocyte differentiation and adiponectin expression by inhibiting AP-1 expression.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-013-2603-6