Rapid detection of mycobacterial contamination in batches of whole cells of purified Mycobacterium leprae by pyrolysis gas chromatography

Preparations of Mycobacterium leprae, purified from host animal tissues, were screened by pyrolysis high‐resolution gas‐chromatographic analysis (Py‐GC) for the presence of (myco)bacterial contaminants, which produce wax‐ester mycolates. Contamination levels as low as 5% of the total number of acid‐...

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Bibliographic Details
Published in:FEMS microbiology letters 1984-01, Vol.25 (2‐3), p.289-293
Main Authors: Wieten, G., Boon, J.J., Groothuis, D.G., Portaels, F., Minnikin, D.E.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
fatty acids
Fundamental and applied biological sciences. Psychology
High‐resolution gas chromatography
Microbiology
mycobacteria
mycobacterium
Mycobacterium leprae
quality control
was ester mycolates
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Summary:Preparations of Mycobacterium leprae, purified from host animal tissues, were screened by pyrolysis high‐resolution gas‐chromatographic analysis (Py‐GC) for the presence of (myco)bacterial contaminants, which produce wax‐ester mycolates. Contamination levels as low as 5% of the total number of acid‐fast bacilli could be detected, based on the presence or absence of the marker compounds: a series of isomeric alkenes. The on‐line pyrolysis capillary GC method provides an easy procedure for rapid quality control of preparations of purified M. leprae.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1984.tb01474.x