Antitumor activity of polysaccharides from Lepista sordida against laryngocarcinoma in vitro and in vivo

• Published in: International journal of biological macromolecules 2013-09, Vol.60, p.235-240
• Main Authors: Miao, Susheng, Mao, Xionghui, Pei, Rong, Miao, Suping, Xiang, Cheng, Lv, Yuanjing, Yang, Xianguang, Sun, Ji, Jia, Shenshan, Liu, Yvpeng
• Format: Article
• Language: English
• Subjects: Agaricales - chemistry; Animals; Antineoplastic Agents - chemistry; Antineoplastic Agents - isolation & purification; Antineoplastic Agents - pharmacology; Antitumor; Bioassay-guided isolation; Carcinoma - drug therapy; Carcinoma - pathology; Cell Line, Tumor; Cell Proliferation - drug effects; Disease Models, Animal; Dose-Response Relationship, Drug; Female; Fungal Polysaccharides - chemistry; Fungal Polysaccharides - isolation & purification; Fungal Polysaccharides - pharmacology; Humans; Laryngeal Neoplasms - drug therapy; Laryngeal Neoplasms - pathology; Laryngocarcinoma; Lepista sordida; Mice; Polysaccharide; Tumor Burden - drug effects; Xenograft Model Antitumor Assays
• ISSN: 0141-8130; 1879-0003
• DOI: 10.1016/j.ijbiomac.2013.05.033

•Four polysaccharides (LSPa1, LSPb1, LSPb2 and LSPc1) were purified from Lepista sordida.•LSPb1, LSPb2 and LSPc1 exhibited more potent in vitro antitumor effect than LSPa1.•LSPc1 evidently suppressed the tumor growth in mice bearing human laryngocarcinoma Hep-2 cells.•The various physicochemical properties resulted in the difference of antitumor activity of four polysaccharides. Four water-soluble polysaccharides (LSPa1, LSPb1, LSPb2 and LSPc1) extracted from the fruiting bodies of Lepista sordida were prepared by DEAE cellulose-52 anion-exchange and Sepharose 6 Fast Flow gel-permeation chromatography. LSPa1 was composed of Glc, Man and Gal with a molar ratio of 3.2: 1.3: 0.6. LSPb1, LSPb2 and LSPc1 were composed of Glc, Man, Gal, Rha and Ara in the proportions of 2.6: 2.1: 0.9: 0.6: 0.3, 1.2: 1.0: 2.3: 0.6: 0.5, 0.2: 0.3: 2.3: 0.3: 2.0, respectively. The high-performance gel-permeation chromatography (HPGPC) analysis showed that the average molecular weight (Mw) of four polysaccharides were approximately 156, 134, 96 and 57kDa, respectively. MTT and lactate dehydrogenase (LDH) assays indicated that three acidic polysaccharides, namely LSPb1, LSPb2 and LSPc1, possessed more potent antiproliferative effect on human laryngocarcinoma Hep-2 cells than neutral polysaccharide LSPa1 in a dose- and time-dependent manner and the effect increased in the order of LSPa1