Combined bioassay-chemical fractionation scheme for the determination and ranking of toxic chemicals in sediments

A protocol for the chemical fractionation of sediments and biological testing of these fractions has been developed. Fractions obtained were directly tested for toxicity by using both the Salmonella typhimurium test and the Panagrellus redivivus test. When applied to sediments from Tobin Lake, Saska...

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Bibliographic Details
Published in:Environmental science & technology 1983-06, Vol.17 (6), p.329-334
Main Authors: Samoiloff, Martin R, Bell, James, Birkholz, Detlef A, Webster, G. R. Barry, Arnott, Evelyn G, Pulak, Rock, Madrid, Alicia
Format: Article
Language:English
Subjects:
Freshwater
Panagrellus redivivus
Salmonella typhimurium
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Summary:A protocol for the chemical fractionation of sediments and biological testing of these fractions has been developed. Fractions obtained were directly tested for toxicity by using both the Salmonella typhimurium test and the Panagrellus redivivus test. When applied to sediments from Tobin Lake, Saskatchewan, this method demonstrated that the major toxic constituents of the sediment were neutral compounds that eluate from Florisil columns by 1:1 hexane-dichloromethane. This most toxic fraction containing none of the priority toxic chemicals. These tests demonstrated agreement between the two biological assay systems.
ISSN:0013-936X
1520-5851
DOI:10.1021/es00112a005