An Adenovirus Vector System Used to Express Polyoma Virus Tumor Antigens

We have used a generalized adenovirus vector system to express the three polyoma tumor (T) antigen proteins under the control of the adenovirus major late promoter. One hybrid virus, Ad-PySVR498, expresses high levels of polyoma middle and small T antigens. A second hybrid virus, Ad-LTSVR545, which...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-03, Vol.82 (5), p.1359-1363
Main Authors: Mansour, Suzanne L., Grodzicker, Terri, Tjian, Robert
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
adenovirus
Adenoviruses
antigen (tumor-associated)
Antigens, Viral, Tumor - genetics
Biological and medical sciences
Biotechnology
cloning vectors
Complementary DNA
DNA
DNA, Recombinant
Fundamental and applied biological sciences. Psychology
gene expression
Gene Expression Regulation
Genes, Viral
Genetic engineering
Genetic technics
Genetic Vectors
HeLa cells
Hybridity
Messenger RNA
Methods. Procedures. Technologies
polyoma virus
Polyomavirus - genetics
Promoter regions
RNA, Messenger - genetics
Tumors
Vectors (cloning, transfer, expression). Insertion sequences and transposons
Viral tumor antigens
Viruses
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Summary:We have used a generalized adenovirus vector system to express the three polyoma tumor (T) antigen proteins under the control of the adenovirus major late promoter. One hybrid virus, Ad-PySVR498, expresses high levels of polyoma middle and small T antigens. A second hybrid virus, Ad-LTSVR545, which contains a cDNA copy of the polyoma A gene, overproduces large T antigen. The T antigens produced are indistinguishable from their authentic polyoma counterparts as determined by immunoprecipitation and partial cleavage by V8 protease. Analysis of polyoma mRNAs encoded by the recombinant viruses showed that they initiate from the adenovirus major late promoter and contain the tripartite leader at their 5′ends. Large T antigen isolated from Ad-LTSVR545-infected cells by immunoaffinity was shown to bind selectively to polyoma DNA sequences that contain the origin of viral DNA replication as well as the sites for transcription initiation.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.5.1359