Immunohistochemical Characterization of Macrophages and Myofibroblasts in Fibrotic Liver Lesions Due to Fasciola Infection in Cattle

Histopathologically, fibrosis in Fasciola-infected cattle livers was characterized by inflammatory cell infiltration, such as eosinophils and macrophages, pseudo-lobule, pseudo-bile ducts and fibrotic bridges separating pseudo-lobules; the fibrotic lesions were developed in the Glisson’s sheath. Pse...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2013, Vol.75(7), pp.857-865
Main Authors: GOLBAR, Hossain M., IZAWA, Takeshi, JUNIANTITO, Vetnizah, ICHIKAWA, Chisa, TANAKA, Miyuu, KUWAMURA, Mitsuru, YAMATE, Jyoji
Format: Article
Language:English
Subjects:
Animals
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
biliary fibrosis
Cattle
Cattle Diseases - parasitology
Cattle Diseases - pathology
Fasciola
Fascioliasis - pathology
Fascioliasis - veterinary
Immunohistochemistry - veterinary
Immunophenotyping - veterinary
Keratin-19
liver
Liver - parasitology
Liver - pathology
macrophages
Macrophages - parasitology
Macrophages - pathology
myofibroblasts
Myofibroblasts - parasitology
Myofibroblasts - pathology
Receptors, Cell Surface - metabolism
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Summary:Histopathologically, fibrosis in Fasciola-infected cattle livers was characterized by inflammatory cell infiltration, such as eosinophils and macrophages, pseudo-lobule, pseudo-bile ducts and fibrotic bridges separating pseudo-lobules; the fibrotic lesions were developed in the Glisson’s sheath. Pseudo-bile ducts consisting of epithelial cells reacted clearly to cytokeratin (CK) 19, indicating cholangiocyte origin. Immunophenotypes of macrophages and myofibroblasts were investigated in the fibrotic livers. Macrophages positive for CD68 (reflecting phagocytosis) and CD163 (representing proinflammatory cytokine production) were increased, and those for CD204 (implying lipid metabolism) and Iba-1 (a calcium-binding protein playing role in chemotaxis) decreased in fibrotic livers compared to control livers. Spindle-shaped myofibroblasts positive for vimentin, desmin and α-smooth muscle actin (α-SMA) increased in the peribiliary connective tissues, although the desmin-positive cells were fewer. In addition to the usefulness of these antibodies for macrophage detection in cattle livers, this study shows that macrophages with different immunophenotypes participate in Fasciola-infected cattle livers, in relation to development of myofibroblasts expressing mainly vimentin and α-SMA.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.12-0536