Cross-Reactivity of the Anti-Human D-dimer Monoclonal Antibody 1C9-6F10 to Canine Fibrin Degradation Products

The cross-reactivity of the anti-human D-dimer antibody 1C9-6F10 to the canine D-dimer was evaluated by western blotting using purified canine fibrinogen, fibrin degradation products (XDPs) and plasma from a dog with suspected disseminated intravascular coagulation (DIC). The antibody cross-reacted...

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Bibliographic Details
Published in:Journal of Veterinary Medical Science 2013, Vol.75(7), pp.963-966
Main Authors: MIURA, Naoki, FURUKAWA, Makoto, MAGARI, Yasuo, MOMOI, Yasuyuki
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
Blotting, Western - veterinary
canine
Cross Reactions - immunology
D-dimer antibody
Disseminated Intravascular Coagulation - immunology
Disseminated Intravascular Coagulation - veterinary
Dog Diseases - immunology
Dogs
fibrin degradation product
Fibrin Fibrinogen Degradation Products - immunology
Humans
Latex Fixation Tests - veterinary
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Summary:The cross-reactivity of the anti-human D-dimer antibody 1C9-6F10 to the canine D-dimer was evaluated by western blotting using purified canine fibrinogen, fibrin degradation products (XDPs) and plasma from a dog with suspected disseminated intravascular coagulation (DIC). The antibody cross-reacted with canine XDPs and plasma from the dog with suspected DIC. A latex agglutination assay using 1C9-6F10 measured the canine XDPs in dogs. In conclusion, the antibody 1C9-6F10 cross-reacted to the canine D-fragment with high specificity, but lower affinity compared with the human D-dimer. The latex agglutination assay using the 1C9-6F10 antibody was used to measure canine plasma D-dimer concentrations.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.12-0270