Isolation and characterization of 39 microsatellite loci in the endangered Japanese loach Leptobotia curta

A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population f...

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Published in:Molecular ecology notes 2008, Vol.8 (1), p.145-148
Main Authors: WATANABE, KATSUTOSHI, TAKESHIMA, HIROHIKO, IWATA, AKIHISA, ABE, TSUKASA, UEHARA, KAZUHIKO, KAKIOKA, RYO, KIHIRA, DAIJIRO, NISHIDA, MUTSUMI
Format: Article
Language:English
Subjects:
Botiinae
Cobitidae
kissing loach
STR
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Summary:A microsatellite-enriched genomic library was obtained for the endangered Japanese loach Leptobotia curta, and 39 dinucleotide markers were successfully isolated and characterized. These markers had between one and nine alleles, with expected heterozygosity ranging from 0 to 0.839, in a population from the Lake Biwa-Yodo River system of Japan. Linkage equilibrium was observed in most loci, and only one locus showed significant deviation from Hardy-Weinberg equilibrium. These microsatellite markers will be useful for genetic diversity studies of wild and captive L. curta populations.
ISSN:1471-8278
1755-098X
1471-8286
1755-0998
DOI:10.1111/j.1471-8286.2007.01902.x