Procollagen I-expressing renin cell precursors

Renin-expressing cells in the kidney normally appear as mural cells of developing preglomerular vessels and finally impose as granulated juxtaglomerular cells in adult kidneys. The differentiation of renin-expressing cells from the metanephric mesenchyme in general and the potential role of special...

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Bibliographic Details
Published in:American journal of physiology. Renal physiology 2013-08, Vol.305 (3), p.F355-F361
Main Authors: Karger, Christian, Kurtz, Felix, Steppan, Dominik, Schwarzensteiner, Ilona, Machura, Katharina, Angel, Peter, Banas, Bernhard, Risteli, Juha, Kurtz, Armin
Format: Article
Language:English
Subjects:
Animals
Cell Lineage
Cells
Collagen
Collagen Type I - biosynthesis
Cytochrome P-450 CYP11B2 - deficiency
Female
Gene expression
Glyceraldehyde-3-Phosphate Dehydrogenases - metabolism
Immunohistochemistry
Juxtaglomerular Apparatus - blood supply
Kidney - cytology
Kidney - metabolism
Kidney Cortex - cytology
Kidney Cortex - metabolism
Kidney diseases
Lac Operon - genetics
Mice
Mice, Knockout
Microscopy, Confocal
Real-Time Polymerase Chain Reaction
Renin - physiology
Stem Cells - metabolism
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Summary:Renin-expressing cells in the kidney normally appear as mural cells of developing preglomerular vessels and finally impose as granulated juxtaglomerular cells in adult kidneys. The differentiation of renin-expressing cells from the metanephric mesenchyme in general and the potential role of special precursor stages in particular is not well understood. Therefore, it was the aim of this study to search for renin cell precursors in the kidney. As an experimental model, we used kidneys of aldosterone synthase-deficient mice, which display a prominent compensatory overproduction of renin cells that are arranged in multilayered perivascular cell clusters. We found that the perivascular cell clusters contained two apparently distinct cell types, one staining positive for renin and another one staining positive for type I procollagen (PC1). It appeared as if PC1 and renin expression were inversely related at the cellular level. The proportion of renin-positive to PC1-positive cells in the clusters was inversely linked to the rate of salt intake, as was overall renin expression. Our findings suggest that the cells in the perivascular cell clusters can reversibly switch between PC1 and renin expression and that PC1-expressing cells might be precursors of renin cells. A few of those PC1-positive cells were found also in adult wild-type kidneys in the juxtaglomerular lacis cell area, in which renin expression can be induced on demand.
ISSN:1931-857X
1522-1466
DOI:10.1152/ajprenal.00079.2013