Quantitative microscopy of the lung: a problem-based approach. Part 2: stereological parameters and study designs in various diseases of the respiratory tract

Design-based stereology provides efficient methods to obtain valuable quantitative information of the respiratory tract in various diseases. However, the choice of the most relevant parameters in a specific disease setting has to be deduced from the present pathobiological knowledge. Often it is dif...

Full description

Saved in:
Bibliographic Details
Published in:American journal of physiology. Lung cellular and molecular physiology 2013-08, Vol.305 (3), p.L205-L221
Main Authors: Mühlfeld, Christian, Ochs, Matthias
Format: Article
Language:English
Subjects:
Acute Lung Injury - pathology
Airway Remodeling
Animals
Asthma - pathology
Emphysema - pathology
Humans
Hypertension
Hypertension, Pulmonary - pathology
Imaging, Three-Dimensional - methods
Knowledge
Lung - cytology
Lung - pathology
Lung diseases
Lung Diseases - pathology
Medical treatment
Mice
Microscopy - methods
Rats
Respiratory diseases
Respiratory tract
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Design-based stereology provides efficient methods to obtain valuable quantitative information of the respiratory tract in various diseases. However, the choice of the most relevant parameters in a specific disease setting has to be deduced from the present pathobiological knowledge. Often it is difficult to express the pathological alterations by interpretable parameters in terms of volume, surface area, length, or number. In the second part of this companion review article, we analyze the present pathophysiological knowledge about acute lung injury, diffuse parenchymal lung diseases, emphysema, pulmonary hypertension, and asthma to come up with recommendations for the disease-specific application of stereological principles for obtaining relevant parameters. Worked examples with illustrative images are used to demonstrate the work flow, estimation procedure, and calculation and to facilitate the practical performance of equivalent analyses.
ISSN:1040-0605
1522-1504
DOI:10.1152/ajplung.00427.2012