Three‐dimensional visualization of dermal skin structure using confocal laser scanning microscopy

Summary The properties and performance of collagen‐based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained wi...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 2013-07, Vol.251 (1), p.14-18
Main Authors: ANTUNES, A.P.M., COVINGTON, A.D., PETFORD, N., MURRAY, T., WERTHEIM, D.
Format: Article
Language:English
Subjects:
Animals
Bovine
Cattle
Collagen
Collagen - analysis
Collagen - ultrastructure
Collagens
Confocal
confocal laser scanning microscopy
dermis
Fibre
H&E
Histocytochemistry - methods
Imaging, Three-Dimensional - methods
immersive 3‐D technology
Lasers
Microscopy
Microscopy, Confocal - methods
Optical Imaging - methods
Scanning microscopy
Skin - chemistry
Skin - ultrastructure
Staining and Labeling - methods
Technological change
Three dimensional
Visualization
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Summary:Summary The properties and performance of collagen‐based materials may be affected by the collagen fibre bundle pattern, orientation and weave. The aim of this study was to develop and apply methods to visualize the dermis using confocal laser scanning microscopy from thin tissue sections stained with haematoxylin and eosin. The data was processed to allow three‐dimensional (3‐D) visualization on a PC and using a 3‐D immersive technology system. The 3‐D visualization of the confocal microscope image stacks allowed the evaluation of the collagen macromolecular structure including the collagen fibre bundles. The methods developed provide a novel way of viewing complex organic structures with further potential applications in the medical field.
ISSN:0022-2720
1365-2818
DOI:10.1111/jmi.12052