Single step coupling for multi-responsive water-based chitin/chitosan magnetic nanoparticles

• Published in: Carbohydrate polymers 2013-09, Vol.97 (2), p.441-450
• Main Authors: Chatrabhuti, Sutima, Chirachanchai, Suwabun
• Format: Article
• Language: English
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Animals; Applied sciences; Biological and medical sciences; Chitin - chemical synthesis; Chitin - chemistry; Chitin whisker; Chitosan - chemical synthesis; Chitosan - chemistry; Colloids; Coupling reaction; Covalent bond; DNA extraction; DNA, Bacterial - isolation & purification; Electricity; Escherichia coli - metabolism; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Magnetic nanoparticles; Magnetite Nanoparticles - chemistry; Magnetite Nanoparticles - ultrastructure; Nanotechnology - methods; Natural polymers; Particle Size; Physicochemistry of polymers; Solubility; Solutions; Solvents; Spectroscopy, Fourier Transform Infrared; Staphylococcus aureus - metabolism; Starch and polysaccharides; Static Electricity; Thermogravimetry; Water - chemistry; Water-soluble chitosan
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2013.04.076

•Coupling of chitin/chitosan with magnetic nanoparticles in aqueous is proposed.•The use of glycidoxypropyltrimethoxy silane allows one step coupling reaction.•The product obtained shows solvent and pH responsiveness.•An external magnetic field initiates alignment of nano-rod structured chitin.•The preliminary study shows a potential DNA extraction application. Coupling of chitin whisker (CTWK) or water-soluble chitosan (WSCS) with magnetic nanoparticles (MAG) via a covalent linkage by using a silane coupling agent in an aqueous system is proposed. The use of the silane coupling agent glycidoxypropyltrimethoxy, having a trimethoxy silane as well as an epoxide functionality, allows one step coupling between CTWK or WSCS and MAG. The MAG derivatives obtained are well dispersed and their colloidal aqueous solutions can be stabilized for days. An external magnetic field initiates alignment of nano-rod structured CTWK–MAG in the direction of the magnetic field. The uniqueness of these two materials is that CTWK–MAG shows solvent polarity responsiveness whereas WSCS–MAG performs pH responsiveness. A model application study on DNA extraction confirms that WSCS–MAG with positively charged at pH 7 and with high surface area performs more Escherichia coli extraction ability for two times as compared to CTWK–MAG.