Osteoblast differentiation and phenotype expressions on chitosan-coated Ti-6Al-4V

•Crosslinked chitosan are covalently bond to medical-grade titanium alloy (Ti64) surface.•Chitosan and alginate- and pectin-crosslinked chitosan coatings were non-toxic.•The coatings were able to swell and release anti-inflammatory drug.•Osteoblasts showed enhanced proliferation and collagen deposit...

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Bibliographic Details
Published in:Carbohydrate polymers 2013-09, Vol.97 (2), p.618-626
Main Authors: Lin, Hsin-Yi, Chen, Jing-Huei
Format: Article
Language:English
Subjects:
Alginate
Alginates - pharmacology
alkaline phosphatase
Alkaline Phosphatase - metabolism
Animals
Anti-Inflammatory Agents - pharmacology
Applied sciences
biocompatibility
Biological and medical sciences
Bone repair
Calcium - metabolism
Cell Differentiation - drug effects
cell proliferation
Cell Proliferation - drug effects
Cell Shape - drug effects
Cell Survival - drug effects
Chitosan
Chitosan - pharmacology
Coated Materials, Biocompatible - pharmacology
coatings
collagen
Collagen Type I - metabolism
contact angle
Cross-Linking Reagents - pharmacology
crosslinking
drugs
Exact sciences and technology
Extracellular Matrix - drug effects
Extracellular Matrix - metabolism
Fluorescence
Glucuronic Acid - pharmacology
Hexuronic Acids - pharmacology
Humans
Materials Testing
Medical sciences
mineralization
Natural polymers
osteoblasts
Osteoblasts - cytology
Osteoblasts - drug effects
Osteoblasts - enzymology
Osteoblasts - ultrastructure
Pectin
pectins
Pectins - pharmacology
Pentoxifylline - pharmacology
Phenotype
Physicochemistry of polymers
Staining and Labeling
Starch and polysaccharides
Surface modification
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
Titanium - pharmacology
Titanium implant
wettability
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Summary:•Crosslinked chitosan are covalently bond to medical-grade titanium alloy (Ti64) surface.•Chitosan and alginate- and pectin-crosslinked chitosan coatings were non-toxic.•The coatings were able to swell and release anti-inflammatory drug.•Osteoblasts showed enhanced proliferation and collagen deposition on the coatings.•Cells had higher ALP activity and Ca deposition on the coatings than on Ti64 Chitosan (C), alginate-crosslinked chitosan (CA), and pectin-crosslinked chitosan (CP) were covalently bonded to Ti-6Al-4V surfaces and tested for their biocompatibility. Compared to the clinically treated Ti-6Al-4V surface (Ti64), C, CA, and CP, had higher contact angles and promoted higher cell proliferation, type I collagen deposition, and mineralization after two weeks (all p
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2013.05.048