Inactivation/reactivation of antibiotic-resistant bacteria by a novel UVA/LED/TiO2 system

• Published in: Water research (Oxford) 2013-09, Vol.47 (13), p.4547-4555
• Main Authors: Xiong, Pei, Hu, Jiangyong
• Format: Article
• Language: English
• Subjects: antibiotic resistance; Antibiotic-resistant bacteria; Applied sciences; bacteria; Catalysis - drug effects; Catalysis - radiation effects; Crystallization; Disinfection; Drug Resistance, Microbial - drug effects; Drug Resistance, Microbial - radiation effects; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - isolation & purification; Escherichia coli - radiation effects; Escherichia coli - ultrastructure; Exact sciences and technology; Inactivation; light intensity; lighting; Magnetics; Microbial Viability - drug effects; Microbial Viability - radiation effects; Periodic illumination; Pollution; Reactivation; scotophase; Titanium - pharmacology; titanium dioxide; Ultraviolet Rays; UVA/LED; Water treatment and pollution
• ISSN: 0043-1354; 1879-2448
• DOI: 10.1016/j.watres.2013.04.056

In this study, an effective photocatalytic disinfection system was established using the newly emerged high power UVA/LED lamp. Crystallizing dish coated with TiO2 was prepared by 32-times impregnation-drying processes, and served as the supporting container for water samples. This study focused on the application of this UVA/LED system for the photocatalytic disinfection of selected antibiotic-resistant bacteria, Escherichia coli ATCC 700891. The disinfection performances were studied under various light intensities and illumination modes. Results show that higher light intensity could reach more significant inactivation of E. coli ATCC 700891. With the same UV dose, log-removal of antibiotic-resistant bacteria decreased with circle time in the studied range, while increased with duty circle. A “residual disinfecting effect” was found in the following dark period for bacteria collected at different phases of photocatalytic process. Residual disinfecting effect was found not significant for bacteria with 30 min periodic illumination. While residual disinfecting effect could kill almost all bacteria after 90 min UV periodic illumination within the following 240 min dark period. •The first application of UVA/LED lamp for bacteria inactivation/reactivation study.•Periodic illumination could affect the disinfection process.•A “residual disinfection effect” was found in the disinfection process.•No photoreactivation/dark-repair was found after the UV illumination.