Linear simian virus 40 DNA fragments exhibit a propensity for rolling-circle replication

A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA befo...

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Bibliographic Details
Published in:Molecular and cellular biology 1985-01, Vol.5 (7), p.1785-1790
Main Authors: Deichaite, I, Laver-Rudich, Z, Dorsett, D, Winocour, E
Format: Article
Language:English
Online Access:Get full text
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Summary:A linear simian virus 40 origin-containing DNA fragment replicated in monkey COS cells, generating tandemly repeated (head-to-tail) structures. Electron microscopy revealed circle-and-tail configurations characteristic of rolling-circle replication intermediates. Circularization of the same DNA before transfection led to a theta type of replication which generated supercoiled DNA molecules.
ISSN:0270-7306