Gelatinases and Their Tissue Inhibitors in a Group of Subjects With Metabolic Syndrome

• Published in: Journal of investigative medicine 2013-08, Vol.61 (6), p.978-983
• Main Authors: Hopps, Eugenia, Lo Presti, Rosalia, Montana, Maria, Noto, Davide, Averna, Maurizio R., Caimi, Gregorio
• Format: Article
• Language: English
• Subjects: Acute coronary syndromes; Age; Biomarkers - blood; Cholesterol; Diabetes; Fasting; Female; Gelatinases - antagonists & inhibitors; Gelatinases - blood; Glucose; Heart attacks; Humans; Hypertension; Male; Matrix Metalloproteinase 2 - blood; Matrix Metalloproteinase 9 - blood; Matrix Metalloproteinase Inhibitors - blood; Metabolic syndrome; Metabolic Syndrome - blood; Metabolic Syndrome - diagnosis; Metabolic Syndrome - enzymology; Middle Aged; Mortality; Oxidative stress; Tissue Inhibitor of Metalloproteinase-1 - blood; Tissue Inhibitor of Metalloproteinase-2 - blood; Weight control
• ISSN: 1081-5589; 1708-8267
• DOI: 10.2310/JIM.0b013e318294e9da

AimTo evaluate matrix metalloproteases (MMP)-2 and MMP-9 and tissue inhibitor of metalloproteases (TIMP)-1 and TIMP-2 in a group of subjects with metabolic syndrome (MS) subdivided according to the presence or absence of diabetes mellitus.MethodsWe examined in 90 subjects (51 men and 39 women) with MS, defined following the International Diabetes Federation criteria, and subsequently subdivided into diabetic subjects (22 men and 11 women) and nondiabetic subjects s (29 men and 28 women), the plasma concentrations of MMP-2 and MMP-9 and of TIMP-1 and TIMP-2 using specific enzyme-linked immunosorbent assay kits.ResultsWe found a significant increase in plasma concentrations of MMP-2, MMP-9, TIMP-1, and TIMP-2 in the whole group of MS subjects (P < 0.001) and in both subgroups of MS subjects with diabetes mellitus (P < 0.001) and without diabetes mellitus (P < 0.001) in comparison with healthy controls. We also noted higher concentrations of all the examined parameters in the MS subjects with diabetes mellitus in comparison with the MS subjects without diabetes mellitus. Matrix metalloproteases and TIMPs showed some significant correlations with body mass index and waist circumference and with metabolic parameters in the whole group of MS subjects.ConclusionAn altered pattern of MMPs and their inhibitors is demonstrated in MS; the presence of diabetes mellitus strongly influences the concentration of MMP and TIMP, contributing probably to the increased cardiovascular risk of MS subjects.