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Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran
Background In the northeast and central parts of Iran, Rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (ZCL). This study used both parasitological and molecular methods to identify Leishmania spp. and their different haplotypes that were circulating...
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| Published in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 2013-09, Vol.107 (9), p.550-557 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
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| creator | Hajjaran, Homa Mohebali, Mehdi Abaei, Mohammad Reza Oshaghi, Mohammad Ali Zarei, Zabih Charehdar, Sorour Mirjalali, Hamed Sharifdini, Meysam Teimouri, Aref |
| description | Background
In the northeast and central parts of Iran, Rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (ZCL). This study used both parasitological and molecular methods to identify Leishmania spp. and their different haplotypes that were circulating in the great gerbil populations in ZCL foci from northeastern Iran.
Methods
A cross-sectional study using microscopy, culturing and molecular methods was conducted to detect Leishmania parasites in 194 live R. opimus in ZCL foci from northeastern Iran during 2010-2011.
Results
Leishmania spp. were found in 38.1% (74/194) of the samples by microscopy and in 41.2% (80/194) by culturing. Small papules and skin thickening on the upper edge of the ears were observed in 25 (12. 9%) of the R. opimus. PCR-RFLP and PCR direct sequencing of internal transcribed spacer 1 (ITS1) rRNA showed similar infection rates for L. major and L. turanica in 60 eligible R. opimus, only one mixed infection containing both L. major and L. turanica was found. Phylogenetic analysis was conducted using the ITS1 sequences of 32 isolates that were successfully aligned by comparison of their base sequences with the ITS1 DNA sequence database using ClustalW and MEGA5. The samples were classified into monophyletic clusters (>97% bootstrap). Six haplotypes were observed for L. major and seven for L. turanica.
Conclusion
In northeast Iran, L. major, and L. turanica naturally circulate in R. opimus, and L. major/L. turanica co-infections also exist. Phylogenetic analysis suggested that Leishmania spp. isolated from R. opimus are not a monophyletic group. |
| doi_str_mv | 10.1093/trstmh/trt060 |
| format | article |
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In the northeast and central parts of Iran, Rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (ZCL). This study used both parasitological and molecular methods to identify Leishmania spp. and their different haplotypes that were circulating in the great gerbil populations in ZCL foci from northeastern Iran.
Methods
A cross-sectional study using microscopy, culturing and molecular methods was conducted to detect Leishmania parasites in 194 live R. opimus in ZCL foci from northeastern Iran during 2010-2011.
Results
Leishmania spp. were found in 38.1% (74/194) of the samples by microscopy and in 41.2% (80/194) by culturing. Small papules and skin thickening on the upper edge of the ears were observed in 25 (12. 9%) of the R. opimus. PCR-RFLP and PCR direct sequencing of internal transcribed spacer 1 (ITS1) rRNA showed similar infection rates for L. major and L. turanica in 60 eligible R. opimus, only one mixed infection containing both L. major and L. turanica was found. Phylogenetic analysis was conducted using the ITS1 sequences of 32 isolates that were successfully aligned by comparison of their base sequences with the ITS1 DNA sequence database using ClustalW and MEGA5. The samples were classified into monophyletic clusters (>97% bootstrap). Six haplotypes were observed for L. major and seven for L. turanica.
Conclusion
In northeast Iran, L. major, and L. turanica naturally circulate in R. opimus, and L. major/L. turanica co-infections also exist. Phylogenetic analysis suggested that Leishmania spp. isolated from R. opimus are not a monophyletic group.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1093/trstmh/trt060</identifier><identifier>PMID: 23868742</identifier><identifier>CODEN: TRSTAZ</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Animals ; Biological and medical sciences ; Cross-Sectional Studies ; Disease Reservoirs - parasitology ; Disease Reservoirs - veterinary ; General aspects ; Gerbillinae - parasitology ; Haplotypes ; Human protozoal diseases ; Infectious diseases ; Iran - epidemiology ; Leishmania - classification ; Leishmaniasis, Cutaneous - parasitology ; Leishmaniasis, Cutaneous - transmission ; Leishmaniasis, Cutaneous - veterinary ; Leshmaniasis ; Medical sciences ; Parasitic diseases ; Phylogeny ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Protozoal diseases ; Rodent Diseases - parasitology ; Rodent Diseases - transmission ; Zoonoses</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 2013-09, Vol.107 (9), p.550-557</ispartof><rights>The Author 2013. Published by Oxford University Press on behalf of Royal Society of Tropical Medicine and Hygiene. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. 2013</rights><rights>2014 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-48a3b5f8ca53dbf43a1e82e79398c9d28caebc14d44a61d6fff853d2dc3d78793</citedby><cites>FETCH-LOGICAL-c356t-48a3b5f8ca53dbf43a1e82e79398c9d28caebc14d44a61d6fff853d2dc3d78793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27632655$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23868742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajjaran, Homa</creatorcontrib><creatorcontrib>Mohebali, Mehdi</creatorcontrib><creatorcontrib>Abaei, Mohammad Reza</creatorcontrib><creatorcontrib>Oshaghi, Mohammad Ali</creatorcontrib><creatorcontrib>Zarei, Zabih</creatorcontrib><creatorcontrib>Charehdar, Sorour</creatorcontrib><creatorcontrib>Mirjalali, Hamed</creatorcontrib><creatorcontrib>Sharifdini, Meysam</creatorcontrib><creatorcontrib>Teimouri, Aref</creatorcontrib><title>Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>Background
In the northeast and central parts of Iran, Rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (ZCL). This study used both parasitological and molecular methods to identify Leishmania spp. and their different haplotypes that were circulating in the great gerbil populations in ZCL foci from northeastern Iran.
Methods
A cross-sectional study using microscopy, culturing and molecular methods was conducted to detect Leishmania parasites in 194 live R. opimus in ZCL foci from northeastern Iran during 2010-2011.
Results
Leishmania spp. were found in 38.1% (74/194) of the samples by microscopy and in 41.2% (80/194) by culturing. Small papules and skin thickening on the upper edge of the ears were observed in 25 (12. 9%) of the R. opimus. PCR-RFLP and PCR direct sequencing of internal transcribed spacer 1 (ITS1) rRNA showed similar infection rates for L. major and L. turanica in 60 eligible R. opimus, only one mixed infection containing both L. major and L. turanica was found. Phylogenetic analysis was conducted using the ITS1 sequences of 32 isolates that were successfully aligned by comparison of their base sequences with the ITS1 DNA sequence database using ClustalW and MEGA5. The samples were classified into monophyletic clusters (>97% bootstrap). Six haplotypes were observed for L. major and seven for L. turanica.
Conclusion
In northeast Iran, L. major, and L. turanica naturally circulate in R. opimus, and L. major/L. turanica co-infections also exist. Phylogenetic analysis suggested that Leishmania spp. isolated from R. opimus are not a monophyletic group.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cross-Sectional Studies</subject><subject>Disease Reservoirs - parasitology</subject><subject>Disease Reservoirs - veterinary</subject><subject>General aspects</subject><subject>Gerbillinae - parasitology</subject><subject>Haplotypes</subject><subject>Human protozoal diseases</subject><subject>Infectious diseases</subject><subject>Iran - epidemiology</subject><subject>Leishmania - classification</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Leishmaniasis, Cutaneous - transmission</subject><subject>Leishmaniasis, Cutaneous - veterinary</subject><subject>Leshmaniasis</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Protozoal diseases</subject><subject>Rodent Diseases - parasitology</subject><subject>Rodent Diseases - transmission</subject><subject>Zoonoses</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkU1v1DAQhi0EokvhyBX5gsSBFDuOvc4RVXxUWoGE4BxNHLsxSuzgcZCWP8VfxG227ZHTHOaZZ2b0EvKSswvOWvEuJ8zzWEpmij0iO673uhKSicdkx5iQVVszcUaeIf5krJZctk_JWS200vum3pG_XyCvCSbqg7Mm-xgohIEu43GK1zbY7A01EyB65w3c9qOjB-txnCF4oLgsF3fD4Zp-G-Pcx_mINC5-XvEtBbokP0M60mTRpt_RJzpGzDeePzGGeLtizRBsXJFO92r0WMQ0xJRHC2XgKkF4Tp44mNC-ONVz8uPjh--Xn6vD109Xl-8PlRFS5arRIHrptAEpht41ArjVtd23otWmHerSsL3hzdA0oPignHO6kPVgxLDXBTsnbzbvkuKv1WLuZo_GTtN2ZscbrlUjlZIFrTbUpIiYrOtOD3ecdTcZdVtG3ZZR4V-d1Gs_2-GevgulAK9PAKCByZW3jccHbq9EraR8uDGuy392_gPIabC0</recordid><startdate>20130901</startdate><enddate>20130901</enddate><creator>Hajjaran, Homa</creator><creator>Mohebali, Mehdi</creator><creator>Abaei, Mohammad Reza</creator><creator>Oshaghi, Mohammad Ali</creator><creator>Zarei, Zabih</creator><creator>Charehdar, Sorour</creator><creator>Mirjalali, Hamed</creator><creator>Sharifdini, Meysam</creator><creator>Teimouri, Aref</creator><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20130901</creationdate><title>Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran</title><author>Hajjaran, Homa ; Mohebali, Mehdi ; Abaei, Mohammad Reza ; Oshaghi, Mohammad Ali ; Zarei, Zabih ; Charehdar, Sorour ; Mirjalali, Hamed ; Sharifdini, Meysam ; Teimouri, Aref</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-48a3b5f8ca53dbf43a1e82e79398c9d28caebc14d44a61d6fff853d2dc3d78793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cross-Sectional Studies</topic><topic>Disease Reservoirs - parasitology</topic><topic>Disease Reservoirs - veterinary</topic><topic>General aspects</topic><topic>Gerbillinae - parasitology</topic><topic>Haplotypes</topic><topic>Human protozoal diseases</topic><topic>Infectious diseases</topic><topic>Iran - epidemiology</topic><topic>Leishmania - classification</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Leishmaniasis, Cutaneous - transmission</topic><topic>Leishmaniasis, Cutaneous - veterinary</topic><topic>Leshmaniasis</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Protozoal diseases</topic><topic>Rodent Diseases - parasitology</topic><topic>Rodent Diseases - transmission</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hajjaran, Homa</creatorcontrib><creatorcontrib>Mohebali, Mehdi</creatorcontrib><creatorcontrib>Abaei, Mohammad Reza</creatorcontrib><creatorcontrib>Oshaghi, Mohammad Ali</creatorcontrib><creatorcontrib>Zarei, Zabih</creatorcontrib><creatorcontrib>Charehdar, Sorour</creatorcontrib><creatorcontrib>Mirjalali, Hamed</creatorcontrib><creatorcontrib>Sharifdini, Meysam</creatorcontrib><creatorcontrib>Teimouri, Aref</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hajjaran, Homa</au><au>Mohebali, Mehdi</au><au>Abaei, Mohammad Reza</au><au>Oshaghi, Mohammad Ali</au><au>Zarei, Zabih</au><au>Charehdar, Sorour</au><au>Mirjalali, Hamed</au><au>Sharifdini, Meysam</au><au>Teimouri, Aref</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>2013-09-01</date><risdate>2013</risdate><volume>107</volume><issue>9</issue><spage>550</spage><epage>557</epage><pages>550-557</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><coden>TRSTAZ</coden><abstract>Background
In the northeast and central parts of Iran, Rhombomys opimus (great gerbil) is the primary reservoir host of zoonotic cutaneous leishmaniasis (ZCL). This study used both parasitological and molecular methods to identify Leishmania spp. and their different haplotypes that were circulating in the great gerbil populations in ZCL foci from northeastern Iran.
Methods
A cross-sectional study using microscopy, culturing and molecular methods was conducted to detect Leishmania parasites in 194 live R. opimus in ZCL foci from northeastern Iran during 2010-2011.
Results
Leishmania spp. were found in 38.1% (74/194) of the samples by microscopy and in 41.2% (80/194) by culturing. Small papules and skin thickening on the upper edge of the ears were observed in 25 (12. 9%) of the R. opimus. PCR-RFLP and PCR direct sequencing of internal transcribed spacer 1 (ITS1) rRNA showed similar infection rates for L. major and L. turanica in 60 eligible R. opimus, only one mixed infection containing both L. major and L. turanica was found. Phylogenetic analysis was conducted using the ITS1 sequences of 32 isolates that were successfully aligned by comparison of their base sequences with the ITS1 DNA sequence database using ClustalW and MEGA5. The samples were classified into monophyletic clusters (>97% bootstrap). Six haplotypes were observed for L. major and seven for L. turanica.
Conclusion
In northeast Iran, L. major, and L. turanica naturally circulate in R. opimus, and L. major/L. turanica co-infections also exist. Phylogenetic analysis suggested that Leishmania spp. isolated from R. opimus are not a monophyletic group.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>23868742</pmid><doi>10.1093/trstmh/trt060</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Cross-Sectional Studies Disease Reservoirs - parasitology Disease Reservoirs - veterinary General aspects Gerbillinae - parasitology Haplotypes Human protozoal diseases Infectious diseases Iran - epidemiology Leishmania - classification Leishmaniasis, Cutaneous - parasitology Leishmaniasis, Cutaneous - transmission Leishmaniasis, Cutaneous - veterinary Leshmaniasis Medical sciences Parasitic diseases Phylogeny Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Protozoal diseases Rodent Diseases - parasitology Rodent Diseases - transmission Zoonoses |
| title | Natural infection and phylogenetic classification of Leishmania spp. infecting Rhombomys opimus, a primary reservoir host of zoonotic cutaneous leishmaniasis in northeast Iran |
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