Abundance of denitrification genes under different peizometer depths in four Irish agricultural groundwater sites

This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater z...

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Bibliographic Details
Published in:Environmental science and pollution research international 2013-09, Vol.20 (9), p.6646-6657
Main Authors: Barrett, Maria, Jahangir, Mohammad M. R., Lee, Changsoo, Smith, Cindy J., Bhreathnach, Niamh, Collins, Gavin, Richards, Karl G., O’Flaherty, Vincent
Format: Article
Language:English
Subjects:
Abundance
Agricultural land
Agriculture
Aquatic Pollution
Aquifers
Atmospheric Protection/Air Quality Control/Air Pollution
Bacteria - classification
Bacteria - genetics
Bacteria - metabolism
Bedrock
Biomass
Climate change
Denitrification
Dissolved organic carbon
Earth and Environmental Science
Ecotoxicology
Emissions
Engineering
Environment
Environmental Chemistry
Environmental engineering
Environmental Health
Environmental studies
Enzymes
Gene expression
Genes
Genes, Bacterial
Groundwater
Groundwater - microbiology
Investigations
Ireland
Laboratories
Land management
Land use planning
Nitrates
Nitric oxide
Nitrification
Nitrogen
Nitrous oxide
Physicochemical properties
Piezometers
Research Article
rRNA 16S
Soil contaminants
Statistical analysis
Subsoils
Surface water
Waste Water Technology
Water depth
Water Management
Water Microbiology
Water pollution
Water Pollution Control
Water table
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Summary:This study examined the relationship between the abundance of bacterial denitrifiers in groundwater at four sites, differing with respect to overlaying land management and peizometer depth. Groundwater was sourced from 36 multilevel piezometers, which were installed to target different groundwater zones: (1) subsoil, (2) subsoil to bedrock interface, and (3) bedrock. The gene copy concentrations (GCCs), as gene copies per liter, for bacterial 16S rRNA genes and the denitrifying functional genes, nirK , nirS , and nosZ , were determined using quantitative polymerase chain reaction assays. The results were related to gaseous nitrogen emissions and to the physicochemical properties of the four sites. Overall, nirK and nirS abundance appeared to show no significant correlation to N 2 O production ( P  = 0.9989; P  = 0.3188); and no significant correlation was observed between nosZ and excess N 2 concentrations ( P  = 0.0793). In the majority of piezometers investigated, the variation of nirK and nirS gene copy concentrations was considered significant ( P  
ISSN:0944-1344
1614-7499
DOI:10.1007/s11356-013-1729-3