Detection of Trace Processes in the Networks of Neurons Cultured on Microelectrode Arrays

We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode ar...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2012-09, Vol.153 (5), p.594-597
Main Authors: Sukhanova, A. L., Mineyeva, O. A., Kiselev, I. I., Burtsev, M. S., Anokhin, K. V.
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Culture Techniques
Electric Stimulation
Fluorescent Antibody Technique
Gene Expression Regulation - physiology
Hippocampus - cytology
Internal Medicine
Laboratory Medicine
Mice
Mice, Inbred BALB C
Microelectrodes
Neuronal Plasticity - physiology
Neurons
Neurons - metabolism
Neurophysiology
Pathology
Proto-Oncogene Proteins c-fos - metabolism
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Summary:We compared the effects of electrical stimulation of primary dissociated neuronal cultures cultured on microelectrode arrays in terms on the expression of c-fos transcriptional factor that is involved in plastic reorganization in neurons. Dissociated hippocampal neurons cultured on multielectrode arrays were exposed to two stimulation protocols: high-frequency and low-frequency stimulations. Expression of c-fos was evaluated using immunofluorescence. Both high-frequency and low-frequency stimulations significantly increased c-fos expression in comparison with non-stimulated control. These findings indicate that c-fos expression can be induced in neuronal cell culture by different types of electrical stimulations and can be used for studying plasticity processes in microphysiological in vitro systems.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-012-1774-9