Distribution of T-2 and HT-2 Toxins in Milling Fractions of Durum Wheat

The effect of processing on mycotoxin content in milling fractions has been investigated in 10 samples of durum wheat contaminated with T-2 and HT-2 toxins at levels ranging from 97 to 5,954 μg/kg (sum of T-2 and HT-2 toxins). Either naturally contaminated samples or samples artificially inoculated...

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Bibliographic Details
Published in:Journal of food protection 2011-10, Vol.74 (10), p.1700-1707
Main Authors: PASCALE, Michelangelo, HAIDUKOWSKI, Miriam, TERESA LATTANZIO, Veronica Maria, SILVESTRI, Marco, RANIERI, Roberto, VISCONTI, Angelo
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cereal and baking product industries
Cereals
Consumer Product Safety
Experiments
Flour - analysis
Food contamination & poisoning
Food Contamination - analysis
Food Handling - methods
Food industries
Food microbiology
Food safety
Food-Processing Industry - methods
Food-Processing Industry - standards
Fundamental and applied biological sciences. Psychology
Fungicides
Fusarium sporotrichioides
Humans
Investigations
Liquid chromatography
Mass spectrometry
Milling industry
Mycotoxins
Pasta
T-2 Toxin - analogs & derivatives
T-2 Toxin - analysis
Toxicity
Toxins
Triticum - chemistry
Triticum aestivum
Wheat
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Summary:The effect of processing on mycotoxin content in milling fractions has been investigated in 10 samples of durum wheat contaminated with T-2 and HT-2 toxins at levels ranging from 97 to 5,954 μg/kg (sum of T-2 and HT-2 toxins). Either naturally contaminated samples or samples artificially inoculated with Fusarium sporotrichioides under field conditions were used. A method based on liquid chromatography-tandem mass spectrometry coupled with immunoaffinity column cleanup was validated in-house for the simultaneous analysis of both toxins in a variety of matrices, including uncleaned wheat, cleaned wheat, screenings, bran, red dog, fine middlings, and semolina. Mean recoveries from samples spiked with T-2 and HT-2 toxins at levels of 100 μg/kg ranged from 85 to 107%, with relative standard deviations (RSDs) lower than 14%. The milling process led to an increase of T-2 and HT-2 toxin contents up to 13- and 5-fold in screenings and bran, respectively, compared with occurrence in the uncleaned wheat; however, an overall reduction of T-2 and HT-2 toxins by 54% (RSD, 20%) and 89% (RSD, 3%) was observed in cleaned wheat and in semolina, respectively.
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X.JFP-11-149