Stimulation of sub-sonic vibration promotes the differentiation of adipose tissue-derived mesenchymal stem cells into neural cells

• Published in: Life sciences (1973) 2012-09, Vol.91 (9-10), p.329-337
• Main Authors: Choi, Yun-Kyong, Cho, Hyunjin, Seo, Young-Kwon, Yoon, Hee-Hoon, Park, Jung-Keug
• Format: Article
• Language: English
• Subjects: adipogenesis; Adipogenesis - physiology; Adipose Tissue - cytology; Adipose Tissue - metabolism; Adipose tissue-derived mesenchymal stem cell; antibodies; astrocytes; Cell Differentiation - physiology; Extracellular Signal-Regulated MAP Kinases - metabolism; gene expression regulation; genes; Humans; Mesenchymal Stromal Cells - metabolism; mitogen-activated protein kinase; Neural differentiation; neurons; Neurons - metabolism; Phosphorylation; Reverse Transcriptase Polymerase Chain Reaction; stem cells; Sub-sonic vibration; Time Factors; Up-Regulation - physiology; Vibration; Western blotting
• ISSN: 0024-3205; 1879-0631
• DOI: 10.1016/j.lfs.2012.07.022

Adipose tissue-derived stem cells (AT-MSCs) have been proposed as a new source for nervous tissue damage due to their capacity of neural differentiation potential including neurons, oligodendrocytes and astrocytes. Recently, many studies have demonstrated that sub-sonic vibration (SSV) is an effective cell differentiation method but there have been no studies on the effect of SSV about AT-MSC differentiation into neural-like cells in vitro. Therefore, we examined the effect of SSV on AT-MSCs to investigate the differentiation potential of neural-like cells. We assessed the changes in AT-MSCs by SSV during 4days at 10, 20, 30 and 40Hz (1.0V). After stimulation, they were analyzed by RT-PCR, Western blot and immunohistological analysis using neural cell type-specific genes and antibodies. Further, to confirm the neural differentiation, we investigated adipogenic genes for RT-PCR analysis. For a mechanism study, we analyzed activation levels in time course of ERK phosphorylation after SSV. After 4-day SSV exposure, we observed morphological changes of AT-MSCs. Further, SSV induced gene/protein levels of neural markers while inhibiting adipogenesis and they were mainly upregulated at 30Hz. In addition, phosphorylated ERK level was increased in a time-dependent manner upon 30Hz SSV for 6h. These results demonstrated that SSV affects AT-MSCs differentiation potential and 30Hz SSV affected neural differentiation on AT-MSCs.