PCAF acetylates Runx2 and promotes osteoblast differentiation

Osteoblasts play a crucial role in bone formation. However, the molecular mechanisms involved in osteoblast differentiation remain largely unclear. Runt-related gene 2 (Runx2) is a master transcriptional factor for osteoblast differentiation. Here we reported that p300/CBP-associated factor (PCAF) d...

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Bibliographic Details
Published in:Journal of bone and mineral metabolism 2013-07, Vol.31 (4), p.381-389
Main Authors: Wang, Chao-Yang, Yang, Shu-Feng, Wang, Zhong, Tan, Jun-Ming, Xing, Shun-Min, Chen, De-Chun, Xu, Sheng-Ming, Yuan, Wen
Format: Article
Language:English
Subjects:
Acetylation
Animals
Cell Differentiation - genetics
Cell Line
Core Binding Factor Alpha 1 Subunit - genetics
Core Binding Factor Alpha 1 Subunit - metabolism
Gene Knockdown Techniques
HEK293 Cells
Humans
Medicine
Medicine & Public Health
Metabolic Diseases
Mice
Original Article
Orthopedics
Osteoblasts - cytology
Osteoblasts - metabolism
Osteogenesis - genetics
p300-CBP Transcription Factors - metabolism
Protein Binding
Transcription, Genetic
Up-Regulation - genetics
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Summary:Osteoblasts play a crucial role in bone formation. However, the molecular mechanisms involved in osteoblast differentiation remain largely unclear. Runt-related gene 2 (Runx2) is a master transcriptional factor for osteoblast differentiation. Here we reported that p300/CBP-associated factor (PCAF) directly binds to Runx2 and acetylates Runx2, leading to an increase in its transcriptional activity. Upregulation of PCAF in MC3T3-E1 cells increases the expression of osteogenic marker genes including a lkaline phosphatase ( ALP ), osteocalcin ( Ocn ), and Osteopontin ( Opn ), and ALP activity was stimulated as well. Consequently, the mineralization of MC3T3-E1 cells was remarkably improved by PCAF. In contrast, PCAF knockdown decreases the mRNA levels of ALP , Ocn , and Opn . ALP activity and the mineralized area were attenuated under PCAF knockdown conditions. These results indicate that PCAF is an important regulator for promoting osteoblast differentiation via acetylation modification of Runx2.
ISSN:0914-8779
1435-5604
DOI:10.1007/s00774-013-0428-y