Diagnosis of tuberculosis infection based on synthetic peptides from Mycobacterium tuberculosis antigen 85 complex

Abstract Aim The laboratory diagnosis of pulmonary tuberculosis (TB) and tuberculous meningitis (TBM) is particularly challenging. The aim of the present work is to develop an immunoassay for the diagnosis of TB infection, using synthetic peptides of antigen (Ag) 85 complex of M. tuberculosis (M tb...

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Bibliographic Details
Published in:Clinical neurology and neurosurgery 2013-06, Vol.115 (6), p.678-683
Main Authors: Kashyap, Rajpal S, Shekhawat, Seema D, Nayak, Amit R, Purohit, Hemant J, Taori, Girdhar M, Daginawala, Hatim F
Format: Article
Language:English
Subjects:
Acyltransferases - immunology
Antigen
Antigen 85 complex
Antigens, Bacterial - immunology
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay
Hospitals
Humans
Immunoassay
Laboratories
Methods
Mycobacterium tuberculosis
Mycobacterium tuberculosis - immunology
Neurology
Neurosurgery
Patients
Peptides
Peptides - chemical synthesis
Peptides - immunology
Proteins
Spectrophotometry, Ultraviolet
Spinal Puncture
Tuberculosis
Tuberculosis, Meningeal - cerebrospinal fluid
Tuberculosis, Meningeal - diagnosis
Tuberculosis, Meningeal - immunology
Tuberculosis, Pulmonary - cerebrospinal fluid
Tuberculosis, Pulmonary - diagnosis
Tuberculosis, Pulmonary - immunology
Tuberculous meningitis
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Summary:Abstract Aim The laboratory diagnosis of pulmonary tuberculosis (TB) and tuberculous meningitis (TBM) is particularly challenging. The aim of the present work is to develop an immunoassay for the diagnosis of TB infection, using synthetic peptides of antigen (Ag) 85 complex of M. tuberculosis (M tb ) H37 Rv. Methods Four peptides (7–10 amino acids long) corresponding to group-specific epitopes of Ag 85 complex of M tb were synthesized. All peptides were evaluated by enzyme-linked immunosorbent assay (ELISA) for immunoreactivity with sera and CSF samples of TB and TBM patients respectively. The diagnostic value of the four peptides was evaluated in both the samples. Results It was observed that Ag 85 peptide 1, 3 and 4 had the highest positive rates in the pulmonary patients; however, Ag 85 peptide 1 and 2 had shown good positivity in the TBM subjects. Conclusions The synthetic peptide based ELISA using Ag 85 complex peptides is a sensitive, specific, rapid and cost effective immunoassay for early diagnosis of pulmonary and extrapulmonary TB. In addition, these synthetic peptides are comparatively easy to produce in a reproducible manner compared with the whole antigen.
ISSN:0303-8467
1872-6968
DOI:10.1016/j.clineuro.2012.07.031