A multi-modal stereo microscope based on a spatial light modulator

Spatial Light Modulators (SLMs) can emulate the classic microscopy techniques, including differential interference (DIC) contrast and (spiral) phase contrast. Their programmability entails the benefit of flexibility or the option to multiplex images, for single-shot quantitative imaging or for simul...

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Bibliographic Details
Published in:Optics express 2013-07, Vol.21 (14), p.16541-16551
Main Authors: Lee, M P, Gibson, G M, Bowman, R, Bernet, S, Ritsch-Marte, M, Phillips, D B, Padgett, M J
Format: Article
Language:English
Subjects:
Equipment Design
Equipment Failure Analysis
Holography - instrumentation
Image Enhancement - instrumentation
Imaging, Three-Dimensional - instrumentation
Lighting - instrumentation
Microscopy - instrumentation
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Summary:Spatial Light Modulators (SLMs) can emulate the classic microscopy techniques, including differential interference (DIC) contrast and (spiral) phase contrast. Their programmability entails the benefit of flexibility or the option to multiplex images, for single-shot quantitative imaging or for simultaneous multi-plane imaging (depth-of-field multiplexing). We report the development of a microscope sharing many of the previously demonstrated capabilities, within a holographic implementation of a stereo microscope. Furthermore, we use the SLM to combine stereo microscopy with a refocusing filter and with a darkfield filter. The instrument is built around a custom inverted microscope and equipped with an SLM which gives various imaging modes laterally displaced on the same camera chip. In addition, there is a wide angle camera for visualisation of a larger region of the sample.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.21.016541