Isolation of a teratocarcinoma stem cell lectin implicated in intercellular adhesion

We have previously identified a cell surface teratocarcinoma stem cell lectin with a fucan/mannan specificity. We now report the purification of the hemagglutinin (lectin) from stem cell conditioned medium by exclusion on a Sepharose 2B column, followed by elution with 0.5 M NaCl from DEAE-cellulose...

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Bibliographic Details
Published in:FEBS letters 1985-04, Vol.183 (2), p.228-231
Main Authors: Grabel, Laura B., Singer, Mark S., Martin, Gail R., Rosen, Steven D.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Adhesion
Cell Line
Chromatography, DEAE-Cellulose
Chromatography, Gel
CMF-PBS, calcium and magnesium-free Dulbecco's phosphate-buffered saline
Electrophoresis, Polyacrylamide Gel
Embryonal Carcinoma Stem Cells
Fluorometry
Fundamental and applied biological sciences. Psychology
Glycoproteins
Hemagglutination Tests
Intercellularadhesion
Lectin
lectins
Lectins - isolation & purification
Neoplastic Stem Cells - analysis
Octoxynol
PAGE, polyacrylamide gel electrophoresis
Polyethylene Glycols
Proteins
stem cells
Stem Cells - analysis
teratocarcinoma
Teratocarcinoma stem cell
Teratoma - analysis
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Summary:We have previously identified a cell surface teratocarcinoma stem cell lectin with a fucan/mannan specificity. We now report the purification of the hemagglutinin (lectin) from stem cell conditioned medium by exclusion on a Sepharose 2B column, followed by elution with 0.5 M NaCl from DEAE-cellulose, providing an overall purification of about 90-fold. When this material was analyzed, by SDS-polyacrylamide gel electrophoresis, a major band of M r 56 000 was consistently observed. Hemagglutination activity was renatured from the gels and localized exclusively to a region of the gel that, as detected by fluorography, contains only the 56-kDa component. This suggested that this polypeptide comprises the lectin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(85)80782-1