Isolation of a teratocarcinoma stem cell lectin implicated in intercellular adhesion

• Published in: FEBS letters 1985-04, Vol.183 (2), p.228-231
• Main Authors: Grabel, Laura B., Singer, Mark S., Martin, Gail R., Rosen, Steven D.
• Format: Article
• Language: English
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Cell Adhesion; Cell Line; Chromatography, DEAE-Cellulose; Chromatography, Gel; CMF-PBS, calcium and magnesium-free Dulbecco's phosphate-buffered saline; Electrophoresis, Polyacrylamide Gel; Embryonal Carcinoma Stem Cells; Fluorometry; Fundamental and applied biological sciences. Psychology; Glycoproteins; Hemagglutination Tests; Intercellularadhesion; Lectin; lectins; Lectins - isolation & purification; Neoplastic Stem Cells - analysis; Octoxynol; PAGE, polyacrylamide gel electrophoresis; Polyethylene Glycols; Proteins; stem cells; Stem Cells - analysis; teratocarcinoma; Teratocarcinoma stem cell; Teratoma - analysis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(85)80782-1

We have previously identified a cell surface teratocarcinoma stem cell lectin with a fucan/mannan specificity. We now report the purification of the hemagglutinin (lectin) from stem cell conditioned medium by exclusion on a Sepharose 2B column, followed by elution with 0.5 M NaCl from DEAE-cellulose, providing an overall purification of about 90-fold. When this material was analyzed, by SDS-polyacrylamide gel electrophoresis, a major band of M r 56 000 was consistently observed. Hemagglutination activity was renatured from the gels and localized exclusively to a region of the gel that, as detected by fluorography, contains only the 56-kDa component. This suggested that this polypeptide comprises the lectin.