Bupropion Reduces the Inflammatory Response and Intestinal Injury Due to Ischemia-Reperfusion

• Published in: Transplantation proceedings 2013-07, Vol.45 (6), p.2502-2505
• Main Authors: Cámara-Lemarroy, C.R, Guzmán-de la Garza, F.J, Cordero-Pérez, P, Alarcón-Galván, G, Ibarra-Hernández, J.M, Muñoz-Espinosa, L.E, Fernández-Garza, N.E
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - pharmacology; Biomarkers - blood; Bupropion - pharmacology; Cytokines - blood; Cytoprotection; Disease Models, Animal; Immunologic Factors - pharmacology; Inflammation - metabolism; Inflammation - pathology; Inflammation - prevention & control; Inflammation Mediators - blood; Intestinal Diseases - blood; Intestinal Diseases - pathology; Intestinal Diseases - prevention & control; Intestines - blood supply; Intestines - drug effects; Intestines - metabolism; Intestines - pathology; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury - blood; Reperfusion Injury - pathology; Reperfusion Injury - prevention & control; Surgery; Time Factors
• ISSN: 0041-1345; 1873-2623
• DOI: 10.1016/j.transproceed.2013.04.010

Abstract Intestinal ischemia-reperfusion (I/R) causes severe organ failure and intense inflammatory responses, which are mediated in part by the cytokine tumor necrosis factor-alpha (TNF-alpha). Bupropion is an antidepressant known to inhibit TNF-alpha production. We sought to examine the protective effects of bupropion on intestinal I/R injury in 15 male Sprague-Dawley rats that were randomized to sham surgery, 45 minutes of intestinal ischemia followed by 180 minutes reperfusion, or bupropion (100 mg/kg) before the intestinal I/R injury. To evaluate the systemic inflammatory response induced by intestinal I/R, we measured serum levels of TNF-alpha, interleukins-1 and -6, lipid peroxidation, and transaminases. Histologic analysis evaluated intestinal injury using the Chiu muscosal injury score. After I/R, Chiu score in control animals was 3.6 ± 1.2 vs 2.6 ± 0.53 in animals that received bupropion ( P  < .05). Bupropion pretreatment reduced intestinal. I/R injury and blunted serum elevations of TNF-alpha (0.96 ± 1.1 ng/mL vs 0.09 ± 0.06 ng/mL, P  < .05) and interleukin-1 (0.53 ± 0.24 ng/mL vs 0.2 ± 0.11 ng/mL, P  < .05). Bupropion in reduced intestinal I/R injury through immunomodulatory machanisms that involve inflammatory cytokines such as TNF-alpha.