Polycell-gel assay of water for spores of Saprolegniaceae (fungi), especially those of the Saprolegnia pathogen of fish

Large aliquot water samples (30 ml) were enriched with nutrients, then gelled in the cold with Polycell (wallpapering paste) and incubated to derive Saprolegniaceae colonies and hence deduced spore counts, from water collections of about 1 litre. The Saprolegnia pathogen of fish was a component of t...

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Bibliographic Details
Published in:Hydrobiologia 1984-07, Vol.114 (3), p.237-248
Main Authors: Willoughby, L. G., Pickering, A. D., Johnson, Hilary G.
Format: Article
Language:English
Subjects:
Saprolegnia
Saprolegniaceae
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Summary:Large aliquot water samples (30 ml) were enriched with nutrients, then gelled in the cold with Polycell (wallpapering paste) and incubated to derive Saprolegniaceae colonies and hence deduced spore counts, from water collections of about 1 litre. The Saprolegnia pathogen of fish was a component of the total recoveries; it was recognised on the basis of its secondary zoospore cyst ornamentation. Windermere water intering The Ferry House fish hatchery gave spore assays of 2 to 28 l super(-1) for the pathogen, a significant component of total Saprolegnia which was 6-73 spores per litre.
ISSN:0018-8158
1573-5117
DOI:10.1007/BF00031875