Establishment and characterization of a cell line developed from the neonate larvae of Papilio demoleus Linnaeus (Lepidoptera: Papilionidae)

A new cell line named RIRI-PaDe, developed from the neonate larvae of Papilio demoleus Linnaeus, was established in modified Grace’s medium supplemented with 20% fetal bovine serum. The cell line was incubated at 28°C and consisted of attached round and short spindle-like cells. The population doubl...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2013-02, Vol.49 (2), p.108-113
Main Authors: Ding, Wei-Feng, Feng, Ying, Zhang, Xin, Li, Xian, Wang, Cheng-Ye
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Autographa californica
Autographa californica multiple nucleopolyhedrovirus
Biomedical and Life Sciences
BIOTECHNOLOGY
Cell Biology
Cell Culture
Cell culture techniques
Cell growth
Cell lines
Cell Proliferation
Cells
chromosome number
Chromosomes - genetics
Cultured cells
cytochrome-c oxidase
Developmental Biology
Electron Transport Complex IV - metabolism
Epithelial cells
fetal bovine serum
Flasks
genes
Humans
Insect genetics
Insect larvae
Insect vectors
Larva - cytology
larvae
Lepidoptera
Lepidoptera - cytology
Life Sciences
neonates
Nuclear polyhedrosis virus
Papilio demoleus
Papilionidae
Primary Cell Culture
Stem Cells
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Summary:A new cell line named RIRI-PaDe, developed from the neonate larvae of Papilio demoleus Linnaeus, was established in modified Grace’s medium supplemented with 20% fetal bovine serum. The cell line was incubated at 28°C and consisted of attached round and short spindle-like cells. The population doubling time was 55 h. The chromosome numbers varied widely from 24 to 136 with a mode of 59 at the 71st passage. Comparison of mitochondrial cytochrome c oxidase subunit I gene of the cell line and neonate larvae confirmed that the cell line was of P. demoleus origin. This cell line was susceptible to the Autographa californica multicapsid nucleopolyhedrovirus and Apocheima cinerarius nucleopolyhedrovirus.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-012-9575-z