The Effect of Iron and Copper as an Essential Nutrient on Mitochondrial Electron Transport System and Lipid Peroxidation in Trichoderma harzianum

Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron–sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2013-08, Vol.170 (7), p.1665-1675
Main Authors: Tavsan, Zehra, Ayar Kayali, Hulya
Format: Article
Language:English
Subjects:
Adenosine diphosphate
ATP
Biochemistry
Biological and medical sciences
Biotechnology
Chemistry
Chemistry and Materials Science
Copper
Copper - pharmacokinetics
Electron Transport Complex I - metabolism
Electron Transport Complex II - metabolism
Electrons
Essential nutrients
Fundamental and applied biological sciences. Psychology
Iron
Iron - pharmacokinetics
Lipid Peroxidation - physiology
Lipids
Mitochondria
Mitochondria - metabolism
Nutrient transport
Peroxidation
Sulfur
Trichoderma - metabolism
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Summary:Iron and copper are essential nutrients for all living organisms as cofactors of many enzymes and play important roles in electron transport system (ETS) enzymes which have heme and iron–sulfur centers. In the present study, ETS enzymes, namely, succinate dehydrogenase (SDH) and cytochrome c oxidase (COX), activities as well as adenine nucleotides and lipid peroxidation (LPO) levels of eukaryotic model Trichoderma harzianum grown in varied concentrations of iron (0–20 mg/l) and copper (0–25 mg/l) mediums have been examined. SDH and COX activities increased up to 10 mg/l of iron. COX and SDH activities increased significantly up to 15 and 1 mg/l of copper, respectively. ATP and ADP levels showed a positive correlation with SDH activity with respect to iron–copper concentrations. The trends of AMP were similar with those of ATP and ADP for iron concentrations, while AMP levels elevated until 5 mg/l of copper. As an indicative marker of membrane damage, LPO levels increased with iron and copper concentration. In conclusion, iron and copper concentrations are of critical importance on activities of the ETS enzymes besides adenine nucleotides and LPO levels by maintenance of this metal homeostasis.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-013-0273-4