Integrated process production and extraction of the fibrinolytic protease from Bacillus sp. UFPEDA 485

• Published in: Applied biochemistry and biotechnology 2013-08, Vol.170 (7), p.1676-1688
• Main Authors: Sales, Amanda Emmanuelle, Souza, Fabiana América Silva Dantas de, Teixeira, J. A., Porto, Tatiana Souza, Porto, Ana L. F.
• Format: Article
• Language: English
• Subjects: ATPS; Bacillus; Bacillus - classification; Bacillus - enzymology; Bacteria; Biochemistry; Biological and medical sciences; Bioreactors - microbiology; Biotechnology; Chemistry; Chemistry and Materials Science; Computer Simulation; Enzymatic activity; Experimental design; Extractive fermentation; Fermentation; Fibrinolytic Agents; Fibrinolytic protease; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbiology; Microorganisms; Models, Biological; PEG/sodium sulfate; Peptide Hydrolases - chemistry; Peptide Hydrolases - isolation & purification; Peptide Hydrolases - metabolism; Polyethylene Glycols - metabolism; Proteases; Science & Technology; Soybeans; Sulfates - metabolism; Systems Integration
• ISSN: 0273-2289; 0273-2289; 1559-0291
• DOI: 10.1007/s12010-013-0306-z

Fibrinolytic proteases are enzymes that degrade fibrin; these enzymes are a promising alternative for thrombolytic therapy, and microorganisms produce them. The aim of this study was to evaluate the optimum conditions for the integrated production and purification of fibrinolytic protease from Bacillus sp. UFPEDA 485. Extractive fermentation was carried out in a culture medium containing soybean flour and by adding polyethylene glycol (PEG) and Na2SO4 according to a 23 experimental design. In all assays, the enzyme preferentially partitioned to the bottom phase (K