Comparative studies of apoptosis in Xenopus laevis and mouse thymoma cell lines

With the use of in vitro methods and cell lines, functional aspects of apoptosis in the Xenopus laevis B3/B7 and mouse EL4 thymoma cell lines are revealed. Moreover, by using information gleaned from digital imaging and immunocytochemistry, changes in locations of key proteins implicated in apoptoti...

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Bibliographic Details
Published in:Alternatives to laboratory animals 2013-07, Vol.41 (3), p.211-218
Main Authors: Johnson, Rachel O, Tittle, Thomas V, Sefchick, Maria P M, Zettergren, Leslie D, Ruben, Laurens N, Clothier, Richard H, Balls, Michael
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Cell Line, Tumor
Immunohistochemistry
Mice
Proto-Oncogene Proteins c-mdm2 - analysis
Resveratrol
Stilbenes - pharmacology
Thymoma - chemistry
Thymoma - drug therapy
Thymoma - pathology
Tumor Suppressor Protein p53 - analysis
Ultraviolet Rays
Xenopus laevis
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Summary:With the use of in vitro methods and cell lines, functional aspects of apoptosis in the Xenopus laevis B3/B7 and mouse EL4 thymoma cell lines are revealed. Moreover, by using information gleaned from digital imaging and immunocytochemistry, changes in locations of key proteins implicated in apoptotic anti-cancer responses, e.g. p53 and Mdm2, are shown. Suggestions are offered as to what these results might mean with respect to the evolutionary conservation of the function and structure of these two molecules and to cancer resistance in amphibians. Finally, studies are described on resveratrol as an anti-cancer therapeutic reagent in the two thymoma cell lines and in normal X. laevis thymocytes.
ISSN:0261-1929
2632-3559
DOI:10.1177/026119291304100304