High-throughput generation of spheroids using magnetic nanoparticles for three-dimensional cell culture

Abstract Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method...

Full description

Saved in:
Bibliographic Details
Published in:Biomaterials 2013-11, Vol.34 (34), p.8555-8563
Main Authors: Kim, Jeong Ah, Choi, Jong-Ho, Kim, Minsoo, Rhee, Won Jong, Son, Boram, Jung, Hyun-Kyo, Park, Tai Hyun
Format: Article
Language:English
Subjects:
3-Dimensional (3-D) cell culture
Advanced Basic Science
Bone Marrow Cells
Cell Culture Techniques - methods
Cell Survival
Computer Simulation
Dentistry
Humans
Magnetic field
Magnetic Fields
Magnetic nanoparticles
Magnetics
Microscopy, Confocal
Nanoparticles - chemistry
Spheroid
Spheroids, Cellular - cytology
Tissue engineering
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract Various attempts have been made to develop three-dimensional (3-D) cell culture methods because 3-D cells mimic the structures and functional properties of real tissue compared with those of monolayer cultures. Here, we report on a highly simple and efficient 3-D spheroid generation method based on a magnetic pin-array system to concentrate magnetic nanoparticle-incorporated cells in a focal direction. This system was comprised only of external magnets and magnetically induced iron pins to generate a concentrated magnetic field for attracting cells in a focused direction. 3-D spheroid generation was achieved simply by adding magnetic nanoparticle-incorporated cells into a well and covering the plate with a magnetic lid. Cell clustering occurred rapidly within 5 min and created more compact cells with time through the focused magnetic force. This system ensured not only reproducible and size-controlled generation of spheroids but also versatile types of spheroids such as random mixed, core–shell, and fused spheroids, providing a very useful tool for various biological applications.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2013.07.056