Direct transition of pGA482:ipt plasmid bearing the cytokinin biosynthesis gene into the cells of phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris by electroporation

This is the first study to perform a direct transfer of the pGA482:ipt plasmid (14.6 kb) bearing the cytokinin biosynthesis gene into the cells of phototrophic purple bacteria Rhodopseudomonas palustris and Rhodobacter sphaeroides by electroporation. The use of this method made it possible to reduce...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2013-07, Vol.451 (1), p.194-197
Main Authors: Serdyuk, O. P., Smolygina, L. D., Chekunova, E. M., Sannikova, E. P., Shirshikova, G. N., Khusnutdinova, A. N., Yartseva, N. V.
Format: Article
Language:English
Subjects:
Bacteria
Biochemistry
Biological and Medical Physics
Biomedical and Life Sciences
Biophysics
Biophysics and Molecular Biology
Biosynthesis
Cytokinins - biosynthesis
Cytokinins - genetics
Electroporation - methods
Gene Expression Regulation, Bacterial - physiology
Genes
Life Sciences
Phototrophic Processes - physiology
Plasmids
Plasmids - administration & dosage
Plasmids - genetics
Protein Engineering - methods
Proteobacteria - physiology
Recombinant Proteins - biosynthesis
Transfection - methods
Transformation, Bacterial - genetics
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Summary:This is the first study to perform a direct transfer of the pGA482:ipt plasmid (14.6 kb) bearing the cytokinin biosynthesis gene into the cells of phototrophic purple bacteria Rhodopseudomonas palustris and Rhodobacter sphaeroides by electroporation. The use of this method made it possible to reduce the pro cess of selection of transformed PPB cells by 10 to 14 days. It is also shown for the first time that not only short DNA fragments but also large plasmids, such as pGA482:ipt, can be directly transferred to PPB cells by electroporation.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672913040078