Histological Effects of Inhaled Corticosteroids and ß2-agonists on Laryngeal Mucosa in an Allergic Rat Model

Objective To constitute an animal model of laryngeal allergy and evaluate the laryngeal effects of inhaled corticosteroids and ß2-agonists on the laryngeal mucosa in an allergic rat model. Study Design Prospective randomized. Setting The Experimental Medical Research Institute (DETAE) at Istanbul Un...

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Bibliographic Details
Published in:Otolaryngology-head and neck surgery 2013-09, Vol.149 (3), p.457-465
Main Authors: Akpinar, Meltem Esen, Tekke, Nihal Seden, Yigit, Ozgur, Ercan, Feriha, Durna, Yusuf, Kiran, Demir
Format: Article
Language:English
Subjects:
Administration, Inhalation
Adrenal Cortex Hormones - administration & dosage
Adrenal Cortex Hormones - pharmacology
Albuterol - administration & dosage
Albuterol - pharmacology
Androstadienes - administration & dosage
Androstadienes - pharmacology
Animals
degranulation
Disease Models, Animal
Fluticasone
Hypersensitivity - drug therapy
Hypersensitivity - immunology
inflammation
inhaled corticosteroids
laryngeal allergy
laryngeal mucosa
Laryngeal Mucosa - drug effects
Laryngeal Mucosa - immunology
mast cells
Mast Cells - immunology
Ovalbumin
Prospective Studies
Random Allocation
Rats
Rats, Wistar
ß2‐agonists
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Summary:Objective To constitute an animal model of laryngeal allergy and evaluate the laryngeal effects of inhaled corticosteroids and ß2-agonists on the laryngeal mucosa in an allergic rat model. Study Design Prospective randomized. Setting The Experimental Medical Research Institute (DETAE) at Istanbul University. Subjects and Methods Wistar Albino rats (n = 32) were sensitized with ovalbumin. Unsensitized rats (n = 8) served as controls. The rats were exposed to aerosolized ovalbumin (1%). On days 28 through 42, every 2 days preceeding ovalbumin exposure, rats were further exposed to aerosolized phosphate buffered saline (n = 8), fluticasone propionate (n = 8), salbutamol (n = 8), and combined salbutamol+fluticasone propionate (n = 8). Inflammatory cell infiltration was graded semi-quantitatively. The quantitative data included mast cell count and degranulation. Ultrathin sections were investigated under transmission electron microscope. Results The simultaneous and pairwise comparison of groups (Kruskal-Wallis) revealed statistically significant difference among groups at supraglottic level (critical P < .05,
ISSN:0194-5998
1097-6817
DOI:10.1177/0194599813495364